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| Effects of Different Bedding Litters on Bacterial Community in Pig Biobed |
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| KeyWord:biobed; bedding litters; bacterial community; DGGE |
| Author Name | Affiliation | | HUAN Hai-lin | Institute of Animal Science, Jiangsu Academy of Agricultural Sciences，Nanjing 210014, China | | YAN Jun-shu | Institute of Animal Science, Jiangsu Academy of Agricultural Sciences，Nanjing 210014, China | | ZHOU Wei-ren | Institute of Animal Science, Jiangsu Academy of Agricultural Sciences，Nanjing 210014, China | | BAI Jian-yong | Institute of Animal Science, Jiangsu Academy of Agricultural Sciences，Nanjing 210014, China | | XU Xiao-ming | Institute of Animal Science, Jiangsu Academy of Agricultural Sciences，Nanjing 210014, China | | FENG Guo-xing | Institute of Animal Science, Jiangsu Academy of Agricultural Sciences，Nanjing 210014, China | | GU Hong-ru | Institute of Animal Science, Jiangsu Academy of Agricultural Sciences，Nanjing 210014, China |
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| Abstract: |
| Previous research has shown that different digestion efficiency of various bedding litters in pig biobeds is linked to bacterial variation. Here we examined both bacterial community structure and predominant bands in biobeds with seven different bedding litters（full sawdust, rice husk, distiller′s grains, mushroom residue, vinegar residue, rice straw and rice husk charcoal） using PCR-DGGE technique. The Shannon-Wiener index and richness had significant differences in different litters, with the highest Shannon-Wiener index found in the distiller′s grains but the lowest in the rice straw. Bacterial community structure in 50% rice husk showed high similarity with that in full sawdust, followed by 50% mushroom residue, while the lowest similarity found in 50% straw. Bacterial strains detected in bedding litters belonged to Arthrobacter sp., Amaricoccu sp., Actinomadura sp., Bacillales sp., Clostridium sp., Escherichia sp., Microbacterium sp., Pseudomonas sp., Rhodococcus sp., Staphylococcus sp. and some unclassified-bacteria. In conclusion, bedding litter composition is an important factor influencing bacterial community structure of biobeds. Rice husk and mushroom residues could partially replace sawdust as bedding materials with little impacts on the microbial community. |
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