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| Community Structure and Abundance of Soil Ammonia-oxidizing Bacteria and Ammonia-oxidizing Archea as Influenced by Insect-resistant Bivalent Transgenic Cotton |
| Received:April 14, 2014 |
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| KeyWord:insect-resistant bivalent transgenic cotton;ammonia-oxidizing bacteria;ammonia-oxidizing archaea;T-RFLP;quantitative real-time PCR |
| Author Name | Affiliation | E-mail | | WU Yuan-feng | Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | LI Gang | Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | gangli1981@gmail.com | | XIU Wei-ming | Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China
College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China | xiuweiming@caas.cn | | JI Guo-zhen | College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China | | | SONG Xiao-long | Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | ZHAO Jian-ning | Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | YANG Dian-lin | Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | |
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| Abstract: |
| The cultivation area of genetically modified(GM) crops has expanded significantly in recent years. However, concerns have been raised over impacts of GM crops on soil ecosystem, especially soil microorganisms. Here, we examined the influence of insect-resistant bivalent transgenic cotton on soil ammonia-oxidizing bacteria(AOB) and ammonia-oxidizing archaea(AOA). Terminal restriction fragment length polymorphism(T-RFLP) was used to evaluate community structure change and qPCR to detect abundance difference. Compared to the control, the population of dominant AOB and AOA did not show significant difference in insect-resistant bivalent transgenic cotton soil, with no changes over the growth season though the ratio of each dominant species population varied in different varieties and at different growth stages of cotton. The Shannon index and Evenness index of AOB had no significant difference between the transgenic cotton soil and its control during the whole growth period. The Shannon index of AOA had similar results, but Evenness index of AOA was significantly lower in the transgenic cotton soil than in the control at the seedling stage(P<0.05), with no difference found at the other growth stages. The abundance of AOB in the transgenic cotton soil was higher at the blooming stage, whereas lower at the other stages, as compared with the control soil. However, AOA had lower abundance in the GM cotton than in the control soil throughout the growing season. Therefore, the insect-resistant bivalent transgenic cotton had no significant impact on the community structure of soil AOB and AOA, but reduced the abundance of AOB and AOA, suggesting potential impacts of transgenic cotton on soil ammonia-oxidizing microorganisms. |
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