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| Expression Analysis of OcsHSP Genes in Testis and Ovary of Oxya chinensis Exposed to Acute Cadmium |
| Received:July 14, 2014 |
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| KeyWord:Oxya chinensis;small heat shock protein;Cd;real-time quantitative PCR;testis;ovary |
| Author Name | Affiliation | E-mail | | KOU Li-hua | Institute of Applied Biology, Shanxi University, Taiyuan 030006, China | | | WU Hai-hua | Institute of Applied Biology, Shanxi University, Taiyuan 030006, China | | | LIU Yao-ming | Institute of Applied Biology, Shanxi University, Taiyuan 030006, China | | | ZHANG Yu-ping | Biology Department of Taiyuan Normal University, Taiyuan 030031, China | | | ZHANG Jian-zhen | Institute of Applied Biology, Shanxi University, Taiyuan 030006, China | | | GUO Ya-ping | School of Life Science, Shanxi University, Taiyuan 030006, China | | | MA En-bo | Institute of Applied Biology, Shanxi University, Taiyuan 030006, China | maenbo2003@sxu.edu.cn |
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| Abstract: |
| Small heat shock proteins(sHSPs) can be induced by environmental stresses. In the present study, we analyzed the mRNA expression patterns of OcHSP19.1, OcHSP19.8, OcHSP20.4, OcHSP20.7, and OcHSP21.1 genes in the testis and ovary of the fifth-instar nymphs of Oxya chinensis exposed to acute cadmium(Cd) toxicity using real-time quantitative PCR. In the testis, 0.16 μg·μL-1 Cd2+ induced the expression of OcHSP19.1 and OcHSP19.8 genes in different treatment times. However, 0.32 μg·μL-1 Cd2+ did not cause significant changes of their expression as compared with the control. When treated with 0.48 μg·μL-1 Cd2+, the expression levels of OcHSP19.1 were higher at 2 h and 6 h but lower at 12 h, and those of OcHSP19.8 were higher at 2 h but lower at 6 h, in comparison with the control. Both reached the highest level at 36 h. In the ovary, 0.16 μg·μL-1 and 0.48 μg·μL-1 Cd2+ did not significantly change OcHSP19.1 and OcHSP21.1 genes in different treatment times, but induced the expression of OcHSP20.7 gene at 2 h, compared with the control. In the treatment by 0.32 μg·μL-1 Cd2+, the expression levels of OcHSP19.1 gene were higher at 6 h and 12 h but lower at 24 h, and the expression levels of OcHSP21.1 were lower at 2 h, 6 h, 12 h, and 24 h, as compared with the control. Both genes reached the highest level at 36 h. The expression levels of OcHSP20.7 gene first increased then declined significantly. The expression levels of OcHSP21.1 gene in the testis and OcHSP20.4 gene in the ovary did not significantly differ from the controls. The results show that the expression patterns of the five OcsHSP genes are related to the Cd concentrations and treatment times. |
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