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| Effects of Insect Non-resistant Transgenic Cottons on Bacterial Community Diversity in Soil |
| Received:December 11, 2014 |
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| KeyWord:transgenic cotton;soil microorganism;PCR-DGGE;bacterial diversity |
| Author Name | Affiliation | E-mail | | ZHAO Yun-li | Agro-Environmental Protection Institute, Ministry of Agriculture
Key Laboratory of Original Agro-Environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-Environment and Agro-product Safety, Tianjin 300191, China
College of Life Sciences, Tianjin Normal University, Tianjin 300387, China | | | LI Gang | Agro-Environmental Protection Institute, Ministry of Agriculture
Key Laboratory of Original Agro-Environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-Environment and Agro-product Safety, Tianjin 300191, China | | | XIU Wei-ming | Agro-Environmental Protection Institute, Ministry of Agriculture
Key Laboratory of Original Agro-Environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-Environment and Agro-product Safety, Tianjin 300191, China | | | DUO Li-an | College of Life Sciences, Tianjin Normal University, Tianjin 300387, China | | | CAO Xuan | Department of Agronomy, Tianjin Agricultural University, Tianjin 300384, China | | | LUO Jun-yu | Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China | | | CUI Jin-jie | Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China | | | YANG Dian-lin | Agro-Environmental Protection Institute, Ministry of Agriculture
Key Laboratory of Original Agro-Environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-Environment and Agro-product Safety, Tianjin 300191, China | | | ZHAO Jian-ning | Agro-Environmental Protection Institute, Ministry of Agriculture
Key Laboratory of Original Agro-Environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-Environment and Agro-product Safety, Tianjin 300191, China | zhaojn2008@163.com |
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| Abstract: |
| There are increasing public concerns over the ecological risks of transgenic plants. Under field conditions, the diversity and composition of bacterial community in soils grown with three insect non-resistant transgenic cottons(high-yield transgenic cotton expressing the RNA recognition motif 2 gene, disease-resistant transgenic cotton expressing the gastrodia antifungal protein and high-quality transgenic cotton expressing the [1-aminocyclopropane-1-carboxylate(ACC)oxidase]) and one conventional cotton CCRI 12(as control) were evaluated at the boll-opening stage by denaturing gradient gel electrophoresis(DGGE). The results showed that planting three transgenic cottons did not show significant effects on Shannon-wiener index(H), evenness(EH) and richness(S) of soil bacteria. High degree of similarity in community structure was observed between transgenic and conventional cottons, indicating no influence of transgenic cottons on bacterial community diversity in the short term. High-yield RRM2 transgenic cotton, disease-resistant GAFP transgenic cotton, high-quality ACO2 transgenic cotton and conventional cotton had 67% similarity in community levels and were thus regarded as one group. According to the sequence analysis of DGGE dominant bands, microorganisms which presented the highest homology belonged to families of Flavobacteria, Bacteriovorax, Segetibacter, alpha proteobacterium, Geobacte, Paenisporosarcina, and Acidobacterium, respectively, and all of them were not cultivatable. |
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