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| Effects of Glycyrrhiza glabra Extract on Biochemical Indices and Immune Function of Jian Carp(Cyprinus carpiovar. Jian) Precision Cut Liver Slices Injured by 2, 3, 7, 8 tetrachlorodibenzo-p-dioxin |
| Received:December 04, 2014 |
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| KeyWord:Glycyrrhiza glabra Extract;2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD);precision cut liver slices;tumor necrosis factor-α;interleukin-1β;Immunoglobulin M |
| Author Name | Affiliation | E-mail | | DU Jin-liang | Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | CAO Li-ping | Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | LIU Ying-juan | Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China | | | JIA Rui | Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China | | | ZHAO Cai-yuan | Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China | | | SHEN Yu-jin | Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China | | | YIN Guo-jun | Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China | yingj@ffrc.cn |
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| Abstract: |
| The hepatoprotective effects of Glycyrrhiza glabra extract(GGE) against TCDD-induced liver injury was evaluated using precision cut liver slices(PCLS). The PCLS were randomly divided into six groups: control group, TCDD model group, GGE group(0.8 mg·mL-1), GGE pre-treatment group, GGE post-treatment group, and GGE pre-and post-treatment group. To PCLS culture system 0.2, 0.4 and 0.8 mg·mL-1 GGE were added before(pre-treatment), after(post-treatment) and both before and after(pre-and post-treatment) exposure to 0.3 μg·L-1 TCDD. The culture supernatants of PCLS were collected for detecting activities of glutamic pyruvic transaminase(GPT), glutamic oxaloacetic transaminase(GOT), and superoxide dismutase(SOD), and contents of malondialdehyde(MDA), tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and immunoglobulin M(IgM). Results showed that the pre-treatment group had relieving effect on liver injury by TCDD. The GGE significantly inhibited the elevation of GOT, GPT, MDA,TNF-α,IL-1β, and IgM, but significantly increased level of superoxide dismutase(SOD). Overall results demonstrate the hepatoprotective effects of GGE and support the use of GGE as a hepatoprotective agent in fish. |
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