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| Impacts of Formaldehyde Stress on Hydrogen Peroxide Accumulation in Guard Cells and Stomatal Aperture and Conductance of Vicia Fabag |
| Received:February 02, 2015 |
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| KeyWord:formaldehyde stress;Vicia faba;guard cell;stomatal aperture;stomatal conductance;hydrogen peroxide;antioxidase |
| Author Name | Affiliation | E-mail | | SUN Hui-qun | Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong 650500, China
Department of Resource and Environment, Anqing Normal College, Anqing 246011, China | | | ZHOU Sheng-en | Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong 650500, China | | | WU Huai-sheng | Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong 650500, China | | | LI Kun-zhi | Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong 650500, China | | | LI Bin | Faculty of Chemistry and Engineering, Kunming University of Science and Technology, Chenggong 650500, China | | | CHEN Li-mei | Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong 650500, China | chenlimeikm@126.com |
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| Abstract: |
| It has showed that formaldehyde(HCHO) concentrations in indoor air have elevated recently. Here we used Vicia faba as a test plant to examine the variations of the stomatal aperture and conductance, hydrogen peroxide content, and the activities of antioxidant enzymes under HCHO stress for 0, 24, 48 h and 72 h. The subcellular localization of H2O2 was also measured by fluorescence microscopy. The activities of catalase(CAT), peroxidase(POD) and ascorbate peroxidase(APX) increased at the beginning but decreased afterward. Under HCHO stress for 72 h, superoxide dismutase(SOD) activity of V. faba leaves raised from 284.52 U·min-1·g-1 FW to 291.44 U·min-1·g-1 FW, resulting in increase of H2O2 content from 1.31 μmol·g-1 FW to 2.39 μmol·g-1 FW. Under 0~48 h of HCHO stress, H2O2 in V. faba leaves was mainly distributed in the cytoplasm of guard cells, whereas H2O2 accumulated in both the cytoplasm and the chloroplast of the guard cells after 72 h. The stomatal aperture and conductance decreased by 46.50% and 78.80%, respectively, after continuous HCHO stress for 72 h. Ascorbic acid(ASA)-daubed experiment confirmed that H2O2 played a critical role in regulating the stomatal aperture and conductance of the plant. This research indicates that H2O2 is localized in guard cells of V. faba leaves under HCHO stress of 0.46～0.72 mg·m-3, which effectively inhibits the stomatal aperture and conductance of V. faba leaves and thus protects plant from HCHO stresses. |
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