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| Mitigation of Autotoxicity Stress of Tomato by Ascorbic Acid |
| Received:January 31, 2015 |
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| KeyWord:Lycopersicon esculentum;substrate extracts;ascorbic acid;autotoxicity;germination;ultra-microstructure |
| Author Name | Affiliation | E-mail | | MA Yan-xia | Vegetable Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China
College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China | | | ZHANG Yu-xin | Vegetable Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China | | | HU Lin-li | College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China | | | LÜ Jian | College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China | | | YU Ji-hua | College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China | yujihua@gsau.edu.cn | | WANG Xiao-wei | Vegetable Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China | wangxw@gsagr.ac.cn |
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| Abstract: |
| Ascorbic acid widely present in many plant tissues is a high abundant and small molecular antioxidant. It plays an important role in protecting plant cells from oxidative damages and stimulating cell division and elongation. This experiment was carried out to explore the effects of exogenous ascorbic acid on resistance of tomato to autotoxicity. Tomato seeds were treated with substrate extracts of 3 year continuous tomato cropped soil, and the effects of exogenous ascorbic acid at different concentrations(0, 0.25, 0.5, 1 mmol·L-1) on the seed germination and plant growth of tomato and the ultrastructure of roots were then investigated. It was found that substrate extracts suppressed seed germination, seedling growth, decreased the activities of superoxide dismutase, peroxidase, and ascorbate peroxidase, and reduced the content of glutathione and ascorbic acid, but increased malondialdehyde content and relative electrolyte leakage. Compared with substrate extracts, treatments with ascorbic acid at 0.25, 0.5 mmol·L-1 and 1 mmol·L-1 increased the seed germination rate, germination energy and germination index, enhanced seedling root and epicotyl, elevated the protective enzyme activity and antioxidant content, decreased malondialdehyde content and relative conductivity. In addition, exogenous ascorbic acid could alleviate harmful effects on root structure. There results indicate that supplying exogenous ascorbic acid could alleviate tomato autotoxicity caused by continuous cropping, with 1 mmol·L-1 having the best effect. |
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