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| Effects of Aqueous Copper on Reactive Oxygen Species Content and Anti-oxidation Capacity of Major Tissues in Portunus trituberculatus |
| Received:January 27, 2015 |
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| KeyWord:Portunus trituberculatus;Cu2+;ROS;SOD;T-AOC |
| Author Name | Affiliation | E-mail | | WANG Li | School of Marine, Ningbo University, Ningbo 315211, China | | | HAN Yan-nan | School of Marine, Ningbo University, Ningbo 315211, China | | | JIN Shan | School of Marine, Ningbo University, Ningbo 315211, China | jinshan@nbu.edu.cn | | Zhao Qing-song | School of Marine, Ningbo University, Ningbo 315211, China | | | CHEN Yin-er | School of Marine, Ningbo University, Ningbo 315211, China | | | WANG Chun-lin | School of Marine, Ningbo University, Ningbo 315211, China | |
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| Abstract: |
| High levels of copper in water can affect aquacultural production. Here an experiment was designed to examine the responses of reactive oxygen species(ROS), superoxide dismutase(SOD) and total anti-oxidation capacity(T-AOC) in gill, hepatopancreas and muscle of Portunus trituberculatus exposed to different concentrations of Cu2+(0, 0.04, 0.4, 2 and 4 mg·L-1) using single factor gradient method of ecology. The ROS level increased with elevated concentrations of Cu2+(2 mg·L-1 and 4 mg·L-1), but was no significant differences(P>0.05)between 0.04 and 0.4 mg·L-1. Compared with control, SOD and T-AOC activities in gill, hepatopancreas and muscle were induced by low concentrations of Cu2+(0.04 and 0.4 mg·L-1), and displayed an inverted V-shape over the experimental period. Furthermore, the activities of SOD and T-AOC in gill, hepatopancreas and muscle showed obvious relationships between concentration-time effects and time-effect. Under high concentrations of Cu2+(2 and 4 mg·L-1), the activity of SOD in gill, hepatopancreas and muscle and the activity of T-AOC in gill were induced during the initial 24 h, but activity of T-AOC in hepatopancreas and muscle were inhibited at the beginning and decreased markedly over time. The present results suggest that exposure to low(<0.04 mg·L-1) and short-term exposure to high Cu2+ concentrations(>2 mg·L-1) would not cause significant oxidative damages, but enhance the antioxidant defense system. Under extended exposure to high Cu2+, however, oxidative damage was obviously increased, decreasing the antioxidation capacity. Antioxidant enzyme and non-enzymatic antioxidants could jointly play a role in the defense system. |
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