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| Characteristics of Lignocellulose Degradation and the Microbial Diversity of FWD1 |
| Received:February 11, 2015 |
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| KeyWord:microbial community;wheat straw;metabolites;bacterial diversity;high-throughput 16S rRNA gene sequencing technology |
| Author Name | Affiliation | E-mail | | ZHAO Ting | College of Sciences, Northwest A&F University, Yangling 712100, China | | | ZHANG Kai-yu | College of Sciences, Northwest A&F University, Yangling 712100, China | | | GU Jie | College of Resources and Environment, Northwest A&F University, Yangling 712100, China
Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture, Yangling 712100, China | | | ZHANG She-qi | College of Sciences, Northwest A&F University, Yangling 712100, China | | | WANG Xiao-juan | College of Resources and Environment, Northwest A&F University, Yangling 712100, China
Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture, Yangling 712100, China | xiaojuan456@163.com | | SONG Wen | College of Sciences, Northwest A&F University, Yangling 712100, China | | | SHI Long-xiang | College of Resources and Environment, Northwest A&F University, Yangling 712100, China
Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture, Yangling 712100, China | | | YAN Fa-wei | College of Resources and Environment, Northwest A&F University, Yangling 712100, China
Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture, Yangling 712100, China | | | PAN Hong-jia | College of Resources and Environment, Northwest A&F University, Yangling 712100, China
Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture, Yangling 712100, China | |
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| Abstract: |
| A microbial community FWD1, which is relatively stable and has an efficient ability to decompose wheat straw, was selected from fir forest soil by using restrictive and successive cultivation method. The degradation rate of wheat straw by FWD1 was as high as 76.92% within 10 days of incubation. The metabolite products of FWD1 were found to be mainly acetic acid, propionic acid, butyric acid and ethanol. The bacterial composition of FWD1 and the fir forest soil sample where FWD1 colonized was characterized by using the next generation of high-throughput 16S rRNA gene sequencing technology. The dominated phyla of FWD1 were Proteobacteria, Firmicutes and Bacteroidetes and the predominated species were Clostridium sp. BNL1100, uncultured Alcaligenes sp., uncultured Alcaligenaceae bacterium and Brevundimonas diminuta. Successive subcultivation led to the enrichment of specific bacterial groups, in phyla of the Proteobacteria and Firmicutes, in species of the Clostridium sp. BNL1100 of cellulolytic bacteria. The stability of the microbial community FWD1 and its functions were maintained by the synergistic coordination among bacterial species in the system. The results provide the basis for a better understanding of the mechanisms of lignocellulose degradation and for the improvement of the efficiency of lignocellulose degradation. |
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