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| Effects of 1,3-Dibromo-5,5-Dimethylhydantoin(DBDMH) on Plasma Antioxidant Defense System in GIFT Nile Tilapia |
| Received:May 22, 2015 |
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| KeyWord:GIFT Nile tilapia;DBDMH;plasma;antioxidant indicators |
| Author Name | Affiliation | E-mail | | SUN Bo | Wuxi Fishery College of Nanjing Agricultural University, Wuxi 214081, China | | | QU Jian-hong | Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | QIU Li-ping | Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | ZHENG Yao | Wuxi Fishery College of Nanjing Agricultural University, Wuxi 214081, China
Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | CHEN Jia-zhang | Wuxi Fishery College of Nanjing Agricultural University, Wuxi 214081, China
Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | chenjz@ffrc.cn |
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| Abstract: |
| 1,3-dibromo-5,5-dimethylhydantoin(DBDMH) is widely used as a disinfectant in aquaculture. However, it may pose toxicity to aquatic organisms. In this study, GIFT Nile tilapias were exposed to different DBDMH concentrations(0, 0.06, 0.3, and 1.5 mg·L-1) for 16 days, followed by a recover period of 10 days. A suit of plasma parameters were detected on days 1, 2, 4, 8, 16 and 26(after 10-day recovery). These parameters were:total-glutathione contents(T-GSH), oxidized glutathione content(GSSG), glutathione-S-transferase activity(GSTs), superoxide dismutase activity(SOD), catalase activity(CAT), glutathione reductase activity(GR), glutathione peroxidase activity(GPx) and total antioxidant capacity activity(T-AOC). Results indicated that SOD and GR activities decreased initially and then maintained stable. T-GSH content, GSH/GSSG ratio, and CAT and GST activities showed initial increase followed by reduction, whereas all these parameters had a significant increment on the day 16. GPx activity displayed a significant rise on day 1 and day 16. T-AOC activity increased only on day 16. The activities of CAT, GPx, T-GSH, and T-AOC and GSH/GSSG ratio revealed a dose-dependent pattern. T-AOC activity showed a dose-effect relationship with DBDMH concentrations. Ten day recovery in water free of DBDNH did not have antioxidant indicators return to the normal levels, except CAT and GR activities. The present study suggests that DBDMH significantly influences the plasma antioxidant defense system of GIFT Nile tilapia and that T-AOC activity can be used as a sensitive biomarker to monitor the DBDMH pollution. DBDMH at high concentrations causes irreversible damages to GIFT Nile tilapia. |
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