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| Genotoxic Effects of Strontium on Root Tip Cells of Vicia faba L. |
| Received:April 09, 2015 |
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| KeyWord:strontium;DNA damage;micronucleus;comet assays;Vicia faba L. |
| Author Name | Affiliation | E-mail | | FU Qian | Life Science College, Sichuan Normal University, Chengdu 610101, China | | | LIU Cong | Life Science College, Sichuan Normal University, Chengdu 610101, China | | | LAI Jin-long | Life Science College, Sichuan Normal University, Chengdu 610101, China | | | WU Guo | Life Science College, Sichuan Normal University, Chengdu 610101, China | | | TAO Zong-ya | Life Science College, Sichuan Normal University, Chengdu 610101, China | t89807596@163.com | | WANG Kun-yan | Life Science College, Sichuan Normal University, Chengdu 610101, China | |
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| Abstract: |
| Strontium(Sr2+) in the environment easily enters plants and poses health risks to human via food pathway. This study was performed to investigate the genotoxic effects of Sr2+ on the root tip cells of broad bean(Vicia faba L.), a Sr2+ high-accumulating and sensitive plant, through acute toxicity test, micronucleus assay, and comet assay. The germination rate, seedling dry weight, chromosome breakage, and DNA damage of broad bean were examined at different Sr2+ concentrations [ρ(Sr2+)0.05~10 mmol·L-1] for 24 h, 48 h, and 72 h, with ρ(Sr2+) 0.01 mmol·L-1 as control(CK). Low concentrations of Sr2+ [ρ(Sr2+)<0.05 mmol·L-1] promoted elongation of the root and shoot of broad bean, but the germination rates were reduced by up to 17.93%~36.32% by ρ(Sr2+)> 0.25 mmol·L-1. As Sr2+ concentrations were ρ(Sr2+) 0.25~10 mmol·L-1, the average biomass and the mitotic index(MI) of the plant decreased, while the chromosomal aberration frequency(CAF), the micronucleus frequency(MCN) and the three parameter values of the comet assay(tail length, tail DNA%, and tail moment) all increased. In conclusion, Sr2+ has a dual effect on root tip cells, depending upon its concentration levels. The toxicity mechanism of Sr2+ on broad bean is structural damages of DNA in root tip cells. |
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