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| Analysis of Differentially Expressed Genes of Amaranthus hypochondriacus Under Cadmium Stress by DDRT-PCR |
| Received:May 24, 2015 |
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| KeyWord:Amaranthus hypochondriacus;cadmium stress;DDRT-PCR |
| Author Name | Affiliation | E-mail | | JIN Hai-jun | College of Life Science, Sichuan Agricultural University, Ya'an 625014, China | | | HU Hong-li | College of Life Science, Sichuan Agricultural University, Ya'an 625014, China | | | CHEN Hui | College of Life Science, Sichuan Agricultural University, Ya'an 625014, China | chen62hui@126.com | | ZHANG Shi-rong | College of Environmental Sciences, Sichuan Agricultural University, Chengdu 611130, China | | | WU Qi | College of Life Science, Sichuan Agricultural University, Ya'an 625014, China | | | SUN Rong | College of Life Science, Sichuan Agricultural University, Ya'an 625014, China | | | TANG Zi-zhong | College of Life Science, Sichuan Agricultural University, Ya'an 625014, China | |
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| Abstract: |
| Amaranthus hypochondriacus has great potential for phytoremediation of cadmium(Cd) polluted soils. In this study, differential display reverse transcriptase polymerase chain reaction(DDRT-PCR) was used to screen differentially expressed genes in the roots, leaves and fruits of A. hypochondriacus under Cd stress. The novel bands were recovered and four differentially expressed cDNA fragments[EST(Expressed sequence tags)-1, EST-2, EST-3 and EST-4] were obtained. All ESTs had homology with known cDNA clones in the Genbank databases. The EST-1 was similar to partial sequences of Talinum paniculatum 26S ribosomal RNA gene(GB|HQ843466.1)with an identity of 99%, while the EST-2 showed similarity to partial sequences of Saxifraga mertensiana large subunit 26S ribosomal RNA gene(GB|AF036498.1) with an identity of 93%. The EST-3 was similar to complete cds of Escherichia coli strain TW14359 DNA polymerase Ⅲ alpha subunit(ECs0186) gene(GB|EU906049.1) and their identity was 97%. The EST-4 was similar to Aspergillus oryzae RIB40 beta-glucosidase A(XM_001816779.2) and the identity was 99%. After BlastX comparison, EST-1 was similar to hypothetical protein MTR_5g051030 [Medicago truncatula](XP_003614386.1) with an identity of 26%, EST-2 similar to putative senescence-associated protein[Cupressus sempervirens](GB|ACA30301.1) with an identity of 98%, EST-3 similar to DNA polymerase Ⅲ subunit alpha[Escherichia coli OP50](ZP_06935700.1) with an identity of 100%, and EST-4 similar to beta-glucosidase[Aspergillus flavus NRRL3357](XP_002383240.1) with an identity of 99%. These four differentially expressed genes were responsive to cadmium stress through regulating protein metabolism, DNA replication and glucose metabolism. |
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