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| Effects of Cattle Farm Effluent Irrigation on Community Structure and Abundance of Ammonia-Oxidizing Bacteria in Soil |
| Received:March 16, 2015 |
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| KeyWord:ammonia-oxidizing bacteria;ammonium-oxidizing archaea;cattle effluent irrigation;community structure;terminal restriction fragment length polymorphism(T-RFLP) |
| Author Name | Affiliation | E-mail | | WANG Ting | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China
Tianjin Normal University, College of Life Sciences, Tianjin 300387, China | | | LIU Li-li | Tianjin Normal University, College of Life Sciences, Tianjin 300387, China | | | ZHANG Ke-qiang | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | SHEN Shi-zhou | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | FENG Jie | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | WANG Feng | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | DU Hui-ying | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | GAO Wen-xuan | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China
Tianjin University, School of Chemical Engineering and Technology, Tianjin 300072, China | wenxuangao@hotmail.com |
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| Abstract: |
| Ammonia-oxidizing bacteria(AOB) and ammonia-oxidizing archaea(AOA) play important roles in the biogeochemical cycle of N element, which is closely related to soil quality, greenhouse gas emissions and NO3- leaching. Therefore, diversity and composition of soil AOB and AOA are valuable indicators for soil quality monitoring. In this study, the effects of five fertilization treatments, including control without fertilizers(CK), conventional fertilization(CF 300 kg N·hm-2) and three irrigation treatments with cattle farm effluent(T4 105 kg N·hm-2, T5 210 kg N·hm-2 and T11 317 kg N·hm-2), on the diversity and composition of AOA and AOB were determined through terminal restriction fragment length polymorphism(T-RFLP) of aomA gene(encoding ammonia monooxygenase). Results showed that the diversity indices, Shannon-Wiener(H') and Pielou(E), of AOB were the highest in the T5 treatment, while they were the lowest in the T11 treatment. Phylogenetic tree analysis indicated that the AOB in the soil mainly belonged to Nitrosospira and Nitrosomanas. However, the indices of Shannon-Wiener(H'), Simpson(Ds) and Pielou(E) of AOA were the highest in the T11 treatment, but the lowest in the CF treatment. AOA aomA gene sequences showed that most sequences of AOA were clustered with Cluster S. The dominant AOB was similar to one species from the rumen of China Holstein cow, which was a clue of community evolution in cattle effluent irrigated farmland. The present results indicate that cattle effluent irrigation could affect the diversity and abundance of soil AOA. |
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