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Acute Toxicity of Single and Co-Exposure of Chromium(Ⅵ) and Phenanthrene to Eisenia Foetida
Received:June 02, 2015  
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KeyWord:chromium;phenanthrene;co-exposure;earthworms;acute toxicity
Author NameAffiliationE-mail
CUI Chun-yan College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China  
SHEN Gen-xiang Shanghai Academy of Environmental Sciences, Shanghai 200233, China shengx@saes.sh.cn 
HU Shuang-qing Shanghai Academy of Environmental Sciences, Shanghai 200233, China  
GU Hai-rong Shanghai Academy of Environmental Sciences, Shanghai 200233, China  
ZHANG Hong-chang Shanghai Academy of Environmental Sciences, Shanghai 200233, China  
CAI Xiang Shanghai Academy of Environmental Sciences, Shanghai 200233, China  
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Abstract:
      In recent years,there has been serious combined pollution of heavy metals and PAHs in soils,causing widespread concerns. The acute toxicity effects of single and co-exposure of chromium(Ⅵ) and phenanthrene on Eisenia foetida were examined with artificial soil test using standard OECD 207. The single exposure test showed that 7 d-LC50 and 14 d-LC50 values of chromium(Ⅵ) to Eisenia foetida were 259.98 mg·kg-1 and 241.13 mg·kg-1, respectively, and those of phenanthrene were 88.01 mg·kg-1 and 60.96 mg·kg-1, correspondingly. The earthworm mortality was obviously related with time and concentrations of the chemical exposure. Phenanthrene had greater toxicity to Eisenia foetida than chromium(Ⅵ) did. The joint acute toxicity of chromium(Ⅵ) and phenanthrene examined under toxic equivalent ratio indicated that the 7 d-LC50 values in the mixture were 151.73 mg·kg-1 and 37.93 mg·kg-1, and the 14 d-LC50 137.69 mg·kg-1 and 34.42 mg·kg-1, for chromium(Ⅵ) and phenanthrene, respectively. The joint effect of chromium(Ⅵ) and phenanthrene on Eisenia foetida was additional joint action based on the isobologram of exposure concentrations and time of chromium(Ⅵ) and phenanthrene.