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| Optimization of fermentation conditions for low-temperature and high-efficiency composite microbial system for corn stover degradation and preliminary development of microbial inocula |
| Received:January 23, 2016 |
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| KeyWord:corn stover;degradation;composite microbial system;fermentation |
| Author Name | Affiliation | E-mail | | HU Hai-hong | Agricultural College, Inner Mongolia Agricultural University, Hohhot 010018, China | | | SUN Ji-ying | Agricultural College, Inner Mongolia Agricultural University, Hohhot 010018, China | nmsunjiying@163.com | | GAO Ju-lin | Vocational and Technical College, Inner Mongolia Agricultural University, Baotou 014100, China | | | WANG Zhen | Agricultural College, Inner Mongolia Agricultural University, Hohhot 010018, China | | | BAO Nao-gan-chao-lu | Agricultural College, Inner Mongolia Agricultural University, Hohhot 010018, China | | | HU Shu-ping | Vocational and Technical College, Inner Mongolia Agricultural University, Baotou 014100, China | | | QING Ge-er | Agricultural College, Inner Mongolia Agricultural University, Hohhot 010018, China | |
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| Abstract: |
| Straw-returning to fields is an effective way to increase soil organic matter content and soil fertility, stimulate soil microbial activity, enhance crop yields and quality, and improve the soil physical and chemical properties. It, therefore, plays a crucial role in promoting agricultural sustainable development. In the spring corn region of northern China, however, maize stover degradation is often slow and is even not fully complete in the fall and winter seasons because of low temperature, which causes wasting of resources. Thus, it is necessary to develop efficient stover-decomposing bacterium agent for low-temperature conditions. A low-temperature and high-efficiency composite microbial system, GF-S72, screened from sawdust and degraded corn stover, was developed for degrading corn stover. Result indicated that the best fermentation was urea of 0.1%, C/N ratio of 20:1, inoculating content of 2%, initial pH of culture medium at 8.0 and liquid medium volume of 18 mL/150 mL at 10℃ for 6 d. The experiment showed that diatomite was the best carrier, and high-efficient composite bacterium agent for degrading corn stover was prepared at low-temperature. The experiment also showed that the highest efficient microbial inoculants of corn stover degradation was at 3:1 of bacterial liquid/carrier, 0.05 g/2 g of microbial inoculants/corn stover ratio, 1.42% of moisture content, 10% of preservation humidity, 15℃ of preservation temperature, and pH 8.2. |
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