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| Screening of a biosurfactant-producing bacterium and biosurfactant characteristics |
| Received:February 21, 2016 |
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| KeyWord:bio-surfactant;polycyclic aromatic hydrocarbons (PAHs);fermentation optimization;solubilization |
| Author Name | Affiliation | E-mail | | SHI Jin-li | College of Resources and Environment Science, Nanjing Agricultural University, Nanjing 210095, China | | | ZHANG Yan-cheng | College of Resources and Environment Science, Nanjing Agricultural University, Nanjing 210095, China | | | ZHANG Li-hao | College of Resources and Environment Science, Nanjing Agricultural University, Nanjing 210095, China | | | ZHOU Jing | College of Resources and Environment Science, Nanjing Agricultural University, Nanjing 210095, China | | | ZHOU Li-na | College of Resources and Environment Science, Nanjing Agricultural University, Nanjing 210095, China | | | LI Hui-xin | College of Resources and Environment Science, Nanjing Agricultural University, Nanjing 210095, China | | | HU Feng | College of Resources and Environment Science, Nanjing Agricultural University, Nanjing 210095, China | | | XU Li | College of Resources and Environment Science, Nanjing Agricultural University, Nanjing 210095, China | xuli602@njau.edu.cn |
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| Abstract: |
| A biosurfactant-producing bacterium was isolated and identified as Pseudomonas aeruginosa 147 by physiological and biochemical characteristics and 16S rDNA sequencing. The fermentation products were assigned to glycolipid by Thin Layer Chromatography(TLC) and Fourier Transform Infared Spectroscopy(FT-IR) analysis. The optimal conditions for the strain to produce biosurfactants were peanut oil as initial carbon source,(NH4)2SO4 as nitrogen source, carbon to nitrogen ratio of 25:1, pH 8, temperature of 30℃, and 5 g·L-1 NaCl. Under these conditions, the surface tension of the fermentation broths could be reduced by 42.08 mN·m-1, compared with the control, and stayed stable for 144 hours. During 108 hours of incubation, the bacterium achieved the maximum biomass(2.63 g·L-1) and had the maximum fermentation product(2.02 g·L-1). The solubilization of different polycyclic aromatic hydrocarbons(PAHs) (fluoranthene, pyrene, benzene[a] pyrene) was increased with the addition of the fermentation products. At the same rates of fermentation products, enhanced solubilization of PAHs with high-ring(benzene[a] pyrene) was lower than that with low-ring(fluoranthene, pyrene). |
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