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| Correlationship between denitrifying bacteria and physicochemical factors in cow manure composting |
| Received:March 01, 2016 |
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| KeyWord:compost;denitrifier;physico-chemical parameter;bacterial community structure;redundancy analysis |
| Author Name | Affiliation | E-mail | | ZHU Li-ping | School of Resources and Environmental Sciences, Northeast Agricultural University, Harbin 150030, China | | | XU Xiu-hong | School of Resources and Environmental Sciences, Northeast Agricultural University, Harbin 150030, China | xuxiuhong@neau.edu.cn | | ZHANG Wen-hao | School of Resources and Environmental Sciences, Northeast Agricultural University, Harbin 150030, China | | | CHENG Li-jun | School of Resources and Environmental Sciences, Northeast Agricultural University, Harbin 150030, China | | | LI Hong-tao | School of Resources and Environmental Sciences, Northeast Agricultural University, Harbin 150030, China | | | WANG Zi-qi | School of Resources and Environmental Sciences, Northeast Agricultural University, Harbin 150030, China | | | HE Ning | School of Resources and Environmental Sciences, Northeast Agricultural University, Harbin 150030, China | |
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| Abstract: |
| In the present paper, the community structure of denitrifying bacteria during cow manure composting process was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE) based on the nosZ gene. The relationship between the changes of denitrifying bacteria community structure and the physic-chemical parameters(temperature, pH, water content, NH4+-N and NO3--N)was evaluated by redundancy analysis(RDA). Results showed that the Shannon-Weaver index of denitrifying bacteria increased during 0~4 d but decreased during 7~29 d of the composting. Based on the sequencing of dominant bands, Alcaligenes were the dominant species during composting. Redundancy analysis(RDA) showed that the community structure of dinitrifying bacteria changed little at the early stage of composting(0~4 d), while it changed dramatically in the maturity stage of composting(23~29 d). Pearson correlation analysis showed that denitrifying bacteria community diversity was extremely significantly positively correlated with water content(r=0.960, P≤0.01), and significantly positively correlated with temperature(r=0.766, P≤0.05). |
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