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| Research of ammonia oxidizing bacterial community structure and its correlation with environmental factors in cow manure composting |
| Received:July 25, 2016 |
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| KeyWord:cow manure composting;PCR-DGGE;Real-time PCR;ammonia oxidizing bacteria;redundancy analysis |
| Author Name | Affiliation | E-mail | | SUN Xue-wei | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | XU Xiu-hong | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | xuxiuhong@neau.edu.cn | | MENG Qing-xin | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | CHENG Li-jun | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | ZHANG Wen-hao | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | MEN Meng-qi | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | XU Ben-shu | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | SUN Yu | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | |
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| Abstract: |
| The dynamic succession of ammonia oxidizing bacterial community structure and quantity were investigated using PCR-DGGE(polymerase chain reaction and denaturing gradient gel electrophoresis) and real-time PCR, the correlations between ammonia oxidizing bacterial community structure and physic-chemical factors were evaluated using redundancy analysis(RDA) in cow manure composting. The results showed that during composting the range of ammonia oxidizing bacteria were 1.62×106~3.8×107 copies·g-1, the amount of ammonia oxidizing bacteria reached the peak on 14 day; DGGE profile analysis showed that the community composition of ammonia oxidizing bacteria obviously varied in different periods of composting. Shannon index of ammonia oxidizing bacteria changed with composting temperature, reaching the maximum(2.671 8) on the 7th day. Phylogenetic analysis showed that ammonia oxidizing bacteria were clustered into two groups, Uncultured Nitrosomonas sp. and Uncultured Nitrosospira sp., which belonged to the β-Proteobacteria. the Uncultured Nitrosospira sp. which accounted for about 60% of ammonia oxidizing bacteria were predominant. RDA showed that ammonia and temperature(P<0.05)effected significantly on the community structure of ammonia oxidizing bacteria. Significant changes of ammonia oxidizing bacterial community structure occurred in cow manure composting, and it is confirmed that the changes of ammonia oxidizing bacterial community structure were affected by environmental factors. |
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