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| Teratogenic effects of embryonic exposure to pretilachlor on the larvae of zebrafish |
| Received:November 08, 2016 |
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| KeyWord:pretilachlor;zebrafish;teratogenic effect;Tbx2;BMP-2;BMP-4;shha;ihha |
| Author Name | Affiliation | E-mail | | LIU Ying | Institute of Environment and Plant Protection, Academy of Tropical Agriculture Sciences of China, Haikou 571101, China | | | JIANG Lei | Institute of Environment and Plant Protection, Academy of Tropical Agriculture Sciences of China, Haikou 571101, China | | | PAN Bo | Institute of Environment and Plant Protection, Academy of Tropical Agriculture Sciences of China, Haikou 571101, China | | | LIN Yong | Institute of Environment and Plant Protection, Academy of Tropical Agriculture Sciences of China, Haikou 571101, China | linyongty@163.com |
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| Abstract: |
| To investigate the teratogenic effect and mechanism of abnormality of embryonic exposure to pretilachlor on the larvae of zebrafish, the malformation phenotype and abnormality rate of the larvae were studied after embryos were esposed to 0.50, 1.00 and 1.50 mg·L-1 pretilachlor from 6 h post fertilization(hpf) to 120 hpf, and also the abnormality mechanism was investigated by testing the gene expression related organ development. The results showed that pretilachlor could induce malformations of larvae, including pericardial cyst,spinal curvature and uninflated swim bladder, and the percentage of three kinds of abnormalities increased with the increase of exposure dose. The pericardial cyst rate were 15.56%(P < 0.05), 25.56%(P < 0.01) and spinal curvature rate were 27.78%(P < 0.01), 35.56%(P < 0.01) respectively after embryo exposed to 1.00, 1.50 mg·L-1 pretilachlor. The uninflated swim bladder rate were 20%(P < 0.05), 37.78%(P < 0.01), 60%(P < 0.01) respectively after embryo exposed to 0.50, 1.00, 1.50 mg·L-1 pretilachlor. mRNA expressions of heart development related gene Tbx2[1.00 mg·L-1(P < 0.01), 1.50 mg·L-1(P < 0.05)], skeleton development related genes BMP-2[1.50 mg·L-1(P < 0.05)], BMP-4[1.00 mg·L-1(P < 0.01), 1.50 mg·L-1(P < 0.01)] and swim bladder development related genes shha[1.50 mg·L-1(P < 0.05)], ihha[1.00 mg·L-1(P < 0.05), 1.50 mg·L-1(P < 0.05)] were reduced significantly after embryos were esposed to pretilachlor. The results deduced that pretilachlor could induce heart, skeleton and swim bladder developmental anomaly by supressing the mRNA expressions of above-mentioned oragan development related gene. |
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