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| Studies on epigenetic diversity variation characteristics of Flaveria bidentis genome DNA of four different geographical distributions |
| Received:September 26, 2016 |
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| KeyWord:Flaveria bidentis;epigenetic diversity;DNA methylation;MSAP |
| Author Name | Affiliation | E-mail | | QUAN Zhi-xing | College of Life Science, Shanxi Agricultural University, Taigu 030801, China | | | TIAN Jia-yuan | Agro-Enviromental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | ZHANG Si-yu | Agro-Enviromental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | HUANGFU Chao-he | Agro-Enviromental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | LIU Hong-mei | Agro-Enviromental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | YANG Dian-lin | Agro-Enviromental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | CHANG Hong | College of Life Science, Shanxi Agricultural University, Taigu 030801, China | | | WANG Hui | Agro-Enviromental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | wanghui03@caas.cn |
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| Abstract: |
| We gathered Flaveria bidentis leaves from four typical invasion areas(HDY, CZX, HSH, and TJJ) and by MSAP method DNA epigenetic diversity and polymorphism variation characteristics as well as the correlation between soil environmental factors and Flaveria bidentis total methylation level were studied. The results showed that a total of 993 MSAP bands were amplified. There were 879 polymorphic loci in the total 993 bands and the percentage of which was 88.52%.The polymorphic loci percentage of Flaveria bidentis species in four different invasion area is higher which is between 84.03% and 92.31%. Gst is 0.07 and there were 7% genetic variations between four different Flaveria bidentis species and 93% of which were in species. Nm is 3.321(>1) which showed that there were extensive and frequent gene exchanges between Flaveria bidentis species. Genetic differentiation between populations was not significant, but there were significant differences in methylation patterns. We guess that the Flaveria bidentis may alter its methylation level to adapt to new environment. MSP and MISP clustering analysis showed that the relationship between TJJ and HDY was closest, which may be related to the soil factors and geographical position of two places. However, SPSS analysis showed that there were no significant correlation(P>0.01) between the methylation level and the soil factors. We speculate that the effect of environment to the epigenetic diversity of Flaveria bidentis was the results of multiple factors rather than a single factor. |
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