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| Effect of N-efficient transgenic rice on diversity and community structure of soil ammonia-oxidizing bacteria |
| Received:January 20, 2017 |
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| KeyWord:N-efficient transgenic rice;ammonia-oxidizing bacteria;diversity;PCR-DGGE |
| Author Name | Affiliation | E-mail | | WEI Lin-lin | Key Laboratory of Original Agro-environment Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | YANG Dian-lin | Key Laboratory of Original Agro-environment Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | HOU Meng-yao | Key Laboratory of Original Agro-environment Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China
College of Resource and Environment, Northeast Agricultural University, Harbin 150030, China | | | NI Tu | Key Laboratory of Original Agro-environment Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | LI Gang | Key Laboratory of Original Agro-environment Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | XIU Wei-ming | Key Laboratory of Original Agro-environment Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | WANG Hui | Key Laboratory of Original Agro-environment Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | ZHAO Jian-ning | Key Laboratory of Original Agro-environment Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | zhaojn2008@163.com |
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| Abstract: |
| Our study investigated the effect of N-efficient transgenic rice of different strains(N-04 and N-08) and its parent on the diversity and community structure of soil ammonia-oxidizing bacteria. Regarding the field plot experiment, we analyzed the effect of the N-efficient transgenic rice on diversity and community structure of soil ammonia-oxidizing bacteria with nitrogenous and non-nitrogenous fertilizer application via a denaturing gradient gel electrophoresis(DGGE) technique. No significant difference in richness index(S) between the N-efficient transgenic rice and Nipp was found. Excluding the elongation stage, N-04 and Nipp had no significant difference in Shannon-Wiener index(H) between nitrogenous and non-nitrogenous fertilizer application. During the elongation, heading, and flowering stages with nitrogenous fertilizer application and the elongation stage without nitrogenous fertilizer application, N-08 had significant effects on the H. Under the conditions of nitrogenous and non-nitrogenous fertilizer application, N-04 had no significant difference on the evenness index En) during all growing stages, and N-08 only had significant difference with regard to Nipp during the elongation stage. The sequence and phylogenetic analysis indicated that N-04 and N-08 had more bands than Nipp, and these bands belonged to Nitrosospira and Nitrosomonas. Therefore, N-efficient transgenic rice had significant effects on the H and En of soil ammonia-oxidizing bacteria during certain rice growing stages, and it was more effective than its parent was for accelerating NH4+ transfer to NO3-. |
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