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| Simultaneous extraction and determination of antibiotics in soils using a QuEChERS-based method and liquid chromatography-tandem mass spectrometry |
| Received:March 01, 2017 |
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| KeyWord:QuEChERS;antibiotics;multi-residue analysis;soil;LC-MS/MS |
| Author Name | Affiliation | E-mail | | MENG Ming-hui | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | HE Ze-ying | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | XU Ya-ping | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | WANG Lu | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | PENG Yi | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | LIU Xiao-wei | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | xwliu2006@163.com |
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| Abstract: |
| The aim of this work was to develop a method, based on QuEChERS(quick, easy, cheap, effective, rugged, and safe), to extract and analyze residues of 20 antibiotics in soil. The developed method included extraction with an ACN:EDTA:McIlvaine buffer, clean-up with a dispersive solid phase extraction(d-SPE) adsorbent using primary secondary amine(PSA) and octadecylsilane(C18), and finally analyte determination using liquid chromatography-tandem mass spectrometry(LC-MS/MS). This work optimized different extraction methods and ratios of clean-up adsorbents to perform at seven spiking levels(2.0, 5.0, 10.0, 20.0, 50.0, 100.0, and 200.0 μg·kg-1) with recoveries ranging from 61.4% to 118.9% with relative standard deviations below 20%(n=5). The limits of quantification(LOQ) of the methods were in the range of 2.0~5.0 μg·kg-1 for most analytes. Linear regression coefficients of greater than 0.990 were obtained. This method was applied to the analysis of real agricultural soil samples, confirming the feasibility of the method. |
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