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| Acute toxicity of cadmium and its effects on lipid peroxidation and DNA damage in “standardized” Anodonta woodiana |
| Received:March 23, 2017 |
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| KeyWord:“standardized” Anodonta woodiana;life stages;cadmium;acute toxicity;biomarker |
| Author Name | Affiliation | E-mail | | CHEN Xiu-bao | Key Laboratory of Fishery Eco-Environment Assessment and Resources Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | LIU Hong-bo | Key Laboratory of Fishery Eco-Environment Assessment and Resources Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | SU Yan-ping | Key Laboratory of Fishery Eco-Environment Assessment and Resources Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | JIANG Tao | Key Laboratory of Fishery Eco-Environment Assessment and Resources Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | YANG Jian | Key Laboratory of Fishery Eco-Environment Assessment and Resources Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | jiany@ffrc.cn |
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| Abstract: |
| This study was undertaken to investigate the toxic effect of waterborne Cd2+ on the freshwater bivalve"standardized" Anodonta woodiana, a unique bioindicator used in the "Freshwater Mussel Watch" project. Glochidia(< 24 h, mean length 0.25 mm), early juveniles(< 5 days, mean length 0.27 mm), and late juveniles(6 months, mean length 50 mm) were exposed to various Cd2+ concentrations for the acute toxicity test. Furthermore, late juveniles or adults(48 months, mean length 85 mm) were used to investigate the temporal variation of lipid peroxidation[malondialdehyde(MDA) content] and DNA damage[Olive tail moment(OTM)] in gills, which are the target organs of Cd2+ accumulation. The results showed that the 24-h median effect concentration(EC50) for glochidia was 0.001 8 mg·L-1, whereas the 96-h EC50 values for early and late juveniles were 0.004 7 mg·L-1 and 4.5 mg·L-1, respectively. MDA content was continuously reduced over time in both the 0.05 mg·L-1 and 0.5 mg·L-1 groups, and was not related to Cd2+ concentration(P>0.05). In contrast, the OTM values increased over time, and were linearly and positively correlated with Cd2+ concentration(P<0.05). These results indicate that glochidia and early juveniles may be used as sensitive test organisms for Cd2+ toxicology research, late juveniles and adults may be suitable for biomonitoring Cd2+ pollution, and OTM is a valuable biomarker for early warning of Cd2+ contamination. |
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