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Comparative study of the degradation efficiency of 2 types of microorganisms on the degradation of corn stalks in solid-state fermentation |
Received:March 10, 2017 |
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KeyWord:solid-state fermentation;corn stalks;Trichoderma harzianum;Bacillus subtilis |
Author Name | Affiliation | E-mail | LI Li-bo | College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China | | REN Xiao-dong | College of Life Sciences, Jilin University, Changchun 130118, China | | DOU Sen | College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China | dousen@tom.com |
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Abstract: |
The efficiency of Trichoderma harzianum and Bacillus subtilis to organic carbon, cellulose, lignin content, cellulase activity, xylanase activity and β-glucosidase activity in the process of solid-state fermentation were studied and compared to discuss the efficiency of different microorganisms on the degradation of corn stalk under the solid-state fermentation during 25 days. The research result showed that the decomposition rate of corn stalk, cellulase activity, xylanase activity and β-glucosidase activity of Trichoderma harzianum and Bacillus subtilis were the highest at 10 to 16 days of fermentation. After 25 days of fermentation, Trichoderma harzianum and Bacillus subtilis corn stalk was degraded by 21.79% and 20.12%. The results showed that the degradation effect of Trichoderma harzianum on corn stalks was not significantly different from that of Bacillus subtilis;the residual amount of straw, straw total organic carbon content and residual straw organic carbon were reduced by 1.67%, 0.26% and 1.80%, compared with Bacillus subtilis treatment, respectively. The cumulative degradation of cellulose, lignin, the cellulase activity, xylanase activity and β-glucosidase activity were increased by 6.99%, 6.54%, 0.7 FPU·mL-1, 0.04 IU·mL-1 and 9.26 IU·mL-1, respectively. The results indicated that corn stalks can be degraded by Trichoderma harzianum and Bacillus subtilis, the degradation efficiency of Trichoderma harzianum on corn stalks was not significantly different from that of Bacillus subtilis. |
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