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| Isolation and characterization of phthalate-degrading bacterial strain, and its application in phthalate-contaminated soil |
| Received:December 07, 2017 |
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| KeyWord:DEHP;Mycobacterium sp.;degradation pathway;contaminated soil |
| Author Name | Affiliation | E-mail | | YANG Jing | School of Environment and Energy, South China University of Technology, Guangzhou 510006, China | | | GUO Chu-ling | School of Environment and Energy, South China University of Technology, Guangzhou 510006, China
The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, China
Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency Disposal, South China University of Technology, Guangzhou 510006, China | clguo@scut.edu.cn | | LIU Sha-sha | School of Environment and Energy, South China University of Technology, Guangzhou 510006, China | | | DANG Zhi | School of Environment and Energy, South China University of Technology, Guangzhou 510006, China
The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, China | | | LU Gui-ning | School of Environment and Energy, South China University of Technology, Guangzhou 510006, China
The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, China | |
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| Abstract: |
| Di(2-ethylhexyl) phthalate(DEHP), one of the most widely used phthalates in China, is easily released into the environment. In the present study, we isolated a high-efficient DEHP-degrading bacterial strain(named ASW6D) enriched from the activated sludge of municipal sewage treatment plant. Based on its morphology and 16S rRNA sequence analysis, the bacterial strain was identified as Mycobacterium sp.. This species can efficiently degrade DEHP at a wide range of temperature(20~40℃) and pH(5~10). The optimal temperature and pH for the growth of ASW6D were 30℃ and 8.0, respectively. Under optimal conditions, ASW6D degraded 500 mg·L-1 DEHP by more than 82.87% within 3 d. Further, ASW6D also utilized dimethyl phthalate(DMP) and dibutyl phthalate(DBP) as carbon sources. The metabolites of DEHP degradation were further analyzed by Gas Chromatography-Mass Spectrometer(GC-MS). The metabolic pathway of DEHP was as follows:initially the side chain of DEHP was shortened by β-oxidation, and then DBP was formed; DBP was then converted to phthalic acid(PA) by hydrolysis. The inoculation of ASW6D into the contaminated soil increased the removal rate of DEHP by 58.67%. This indicates that ASW6D has the potential for bioremediation of phthalate acid ester(PAE)-contaminated soil. |
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