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Isolation, identification, and characterization of atrazine-degrading bacterial strain CS3 |
Received:October 25, 2017 |
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KeyWord:atrazine;Arthrobacter sp.;biodegradation |
Author Name | Affiliation | E-mail | YANG Xiao-yan | Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Science, Beijing 100081, China | | LI Yan-ling | Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Science, Beijing 100081, China | | WEI Huan-yu | Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Science, Beijing 100081, China | | ZHU Chang-xiong | Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Science, Beijing 100081, China | | LI Feng | Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Science, Beijing 100081, China | | GENG Bing | Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Science, Beijing 100081, China | gengbing2000@126.com |
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Abstract: |
Isolation of microbial resources from polluted environments used for soil remediation will be increasingly necessary in future agriculture. In view of this, atrazine-degrading bacterial strain CS3, which grows using atrazine as its sole nitrogen source, was isolated from the effluent of a pesticide factory in Hebei Province. Based on its biochemical characterization and 16S rRNA gene sequence, strain CS3 was identified as Arthrobacter unreafaciences. Its optimum growth temperature and pH were 30℃ and 7.0, respectively. Under these conditions, A. unreafaciences CS3 completely degraded 50 mg·L-1 atrazine within 48 h and 500 mg·L-1 within 6 days. A. unreafaciences CS3 contains the atrazine-degrading genes trzN, atzB, and atzC and has good growth and atrazine degradation in the pH range 5~11. |
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