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| Effect of uranium pollution on the soil fungal community as revealed via high-throughput sequencing |
| Received:February 26, 2018 |
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| KeyWord:uranium pollution;fungal community structure;high-throughput sequencing;uranium-resistant fungi |
| Author Name | Affiliation | E-mail | | XIAO Shi-qi | College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China | | | SONG Shou | College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China | | | CHEN Xiao-ming | College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
Defense Key Laboratory, Southwest University of Science and Technology, Mianyang 621010, China | chenxiaoming@swust.edu.cn | | QI Xin | College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China | | | ZHANG Xiang-hui | College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China | | | TIAN Jia | College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China | | | HUANG Sen | College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China | | | DONG Fa-qin | College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China | |
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| Abstract: |
| In order to investigate the effect of uranium pollution on the community structure of soil fungi, in this study, indoor pollution by uranium of the soil environment(2, 5, 10, 20, 50 mg·kg-1 and 100 mg·kg-1)was simulated, and untreated soil samples were blank controls. Using a combination of Illumina MiSeq high-throughput sequencing and bioinformatics analysis method, the diversity and community structure of soil fungi under the different concentrations of uranium were determined. The results showed that uranium had an important influence on fungal diversity. When the uranium concentration was 20 mg·kg-1, the fungal diversity was the lowest. At the phylum level, the dominant strains were from the Zygomycota, accounting for 33.46%~73.36%, with Mortierella the dominant genus, accounting for 33.46%~73.30%. Principal component analysis showed that the fungal community structure of the soil under uranium stress was significantly different from that of the control group. Ten species of uranium-resistant fungi were found via correlation analysis, such as Pseudeurotium, Glomus, and Cylindrocarpon. The results of this study can provide a theoretical basis for evaluating and remediating ecological environments polluted by uranium. |
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