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| The impact of earthworm (Eisenia fetida)on the microbial community in an acetochlor contaminated soil |
| Received:April 17, 2018 Revised:May 24, 2018 |
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| KeyWord:soil;acetochlor;earthworm;microbial community |
| Author Name | Affiliation | E-mail | | HAO Yue-qi | Innovation Team of Soil Organic Contaminant Control, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Original Agro-Environment and Agro-Product Safety, Tianjin 300191, China | | | SUN Yang | Innovation Team of Soil Organic Contaminant Control, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Original Agro-Environment and Agro-Product Safety, Tianjin 300191, China | | | LI Xiao-jing | Innovation Team of Soil Organic Contaminant Control, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Original Agro-Environment and Agro-Product Safety, Tianjin 300191, China | | | ZHOU Bin | Innovation Team of Soil Organic Contaminant Control, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Original Agro-Environment and Agro-Product Safety, Tianjin 300191, China | | | WENG Li-ping | Innovation Team of Soil Organic Contaminant Control, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Original Agro-Environment and Agro-Product Safety, Tianjin 300191, China | | | LI Yong-tao | College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China | | | ZHAO Li-xia | Innovation Team of Soil Organic Contaminant Control, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Original Agro-Environment and Agro-Product Safety, Tianjin 300191, China | zhaolixia@caas.cn |
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| Abstract: |
| In order to investigate the effects of the earthworm (Eisenia fetida)on the microbial community in acetochlor-polluted soil, soil enzyme activity measurement, traditional plate count, and high-throughput sequencing methods were conducted in the present study. Three groups were set up:natural soil (S), soil with acetochlor (SA), and soil with acetochlor and earthworm (SAE). The results showed that 5 mg·kg-1 of acetochlor (recommended field dosage)decreased catalase, dehydrogenase, and invertase activities; and increased urease and alkaline phosphatase activities. The earthworm restored the catalase, dehydrogenase, and invertase activities by 4%, 14%, and 53%, respectively on the 14th day of incubation, which was the most obvious period of the earthworm's effect. The stimulation of the earthworm of bacterial population was transient (the third day of incubation), while its effect on fungi population was long-term (the whole thirty days of incubation). The earthworm repaired the damage to bacterial and fungal populations caused by acetochlor. On day 3 and 14, the bacterial and fungal populations were promoted 35% and 39%, respectively, by the earthworm in the SAE group. The data on soil enzyme activities and microorganism population indicated that the earthworm can repair the ecological damage caused by acetochlor. The soil in the earthworm-containing group (SAE)was characterized as having more neutral pH and higher dissolvable organic carbon content, which provided a more suitable environment for microorganisms. At the phylum level, Proteobacteria, Actinobacteria, and Chytridiomycota were the main phyla inhibited by acetochlor, while Acidobacteria and Zygomycota were stimulated by acetochlor. The earthworm changed the microbial community composition in acetochlor-contaminated soil, making it more similar to that of unspiked soil. The results further illustrate the microbial recovery effect of the earthworm on acetochlor-contaminated soil. At the genus level, a few species like Sphingomonas, Fusarium, Gaertneriomyces, and Pyrenula, promoted by acetochlor, could be the microorganisms involved in acetochlor degradation. |
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