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| Relationship between expression levels of critical genes and Pb uptake and translocation by tomato exposed to Fe-deficiency, Ca-deficiency, and salinity stress |
| Received:March 02, 2018 |
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| KeyWord:tomato;lead uptake;stress;CNGC1;cation channel;HMA3 |
| Author Name | Affiliation | E-mail | | YE Han-jie | School of Environment, Jinan University, Guangzhou 510632, China | | | WANG Li-li | School of Environment, Jinan University, Guangzhou 510632, China | | | YU Dan-ping | School of Environment, Jinan University, Guangzhou 510632, China | | | LI Qu-sheng | School of Environment, Jinan University, Guangzhou 510632, China | liqusheng@21cn.com | | XU Zhi-min | School of Environment, Jinan University, Guangzhou 510632, China | | | ZHOU Chu | School of Environment, Jinan University, Guangzhou 510632, China | | | LIN Xin | School of Environment, Jinan University, Guangzhou 510632, China | | | LÜ Yao | School of Environment, Jinan University, Guangzhou 510632, China | | | ZHOU Ting | School of Environment, Jinan University, Guangzhou 510632, China | |
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| Abstract: |
| A hydroponic experiment was conducted to investigate the characteristics of lead(Pb) accumulation in tomato(Lycopersicon esculentum Mill) exposed to Fe-deficiency, Ca-deficiency, and salinity stress. The genes HMA3 and CNGC1 were located by homology comparison, and their gene expression levels in roots were determined. These results were used to discuss the effect of these two gene expressions on Pb accumulation. The results showed that:(1) Compared with control groups, the Ca-deficiency treatment significantly increased Pb uptake by roots, but inhibited root-to-shoot Pb translocation. The Fe-deficiency treatment significantly increased the amount of Pb translocation from roots to shoots but did not significantly influence root Pb uptake. The salinity treatment significantly enhanced the uptake and transportation of Pb.(2) A very significant positive correlation between Pb uptake by per unit root mass and activity level of CNGC1 cation channel was observed in the three treatments, indicating that CNGC1 cation channel played an important role in Pb uptake. Furthermore, there was a negative correlation between the expression of HMA3 and Pb translocation from roots to shoots, implying that high expression of HMA3 restricted Pb transport to the shoots. Hence, Fe-deficiency, Ca-deficiency, and salinity stress have different effects on Pb accumulation in tomato, and expression levels of HMA3 and CNGC1 control the distribution of Pb in tomato. |
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