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| Selenium accumulation and its effects on the antioxidant system of zebrafish(Danio rerio)exposed to selenite and selenate |
| Received:March 05, 2018 |
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| KeyWord:zebrafish;antioxidant system;catalase(CAT);total antioxidant capacity(T-AOC);Na+/K+-ATPase |
| Author Name | Affiliation | E-mail | | MA Shan-shan | Shenyang Academy of Environmental Sciences, Shenyang 110167, China
Liaoning Provincial Key Laboratory for Urban Ecology, Shenyang 110167, China
Key Laboratory of Clean Combustion for Electricity Generation and Heat-supply Technology of Liaoning Province, Shenyang Institute of Engineering, Shenyang 110136, China
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | | | ZHAO Guang-hui | Shenyang Academy of Environmental Sciences, Shenyang 110167, China
Liaoning Provincial Key Laboratory for Urban Ecology, Shenyang 110167, China | | | CHANG Wen-yue | Shenyang Academy of Environmental Sciences, Shenyang 110167, China
Liaoning Provincial Key Laboratory for Urban Ecology, Shenyang 110167, China | changwenyue@163.com | | LIU Zhi | Shenyang Academy of Environmental Sciences, Shenyang 110167, China
Liaoning Provincial Key Laboratory for Urban Ecology, Shenyang 110167, China | | | ZHANG Yi-ran | Shenyang Academy of Environmental Sciences, Shenyang 110167, China
Liaoning Provincial Key Laboratory for Urban Ecology, Shenyang 110167, China | | | CHEN Hong-xing | The Environmental Research Institute, MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China | | | XIE Ling-tian | The Environmental Research Institute, MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China | |
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| Abstract: |
| This study aimed to explore the effects of Se(Ⅳ) and Se(Ⅵ) exposure on the antioxidant system and Se accumulation in zebrafish(Danio rerio) tissues. The study organisms were divided into three groups, i.e., control group, Se(Ⅳ) -exposed group(500 μg·L-1), and Se(Ⅵ) -exposed group(5000 μg·L-1). The Se accumulation, catalase(CAT), total antioxidant capacity(T-AOC), and Na+/K+-ATPase activity were determined in the brain, gill, liver, intestine, and muscle tissues of zebrafish after exposure to both Se species for 28 days. The results showed different extents of Se accumulation in fish tissues from the Se-exposed groups compared with the control treatment. The accumulation of Se in the liver was the highest. CAT activity in the intestine, T-AOC activity in the gills and intestine, and Na+/K+-ATPase activity in the gills and liver tissues were significantly decreased. As compared with the Se(Ⅵ) treatment, Se accumulation in the liver and gill tissues of fish was significantly elevated in the Se(Ⅳ) treatment. CAT activity in the liver was significantly decreased 13%. However, the T-AOC was significantly increased 1.8 times. These results demonstrated that Se(Ⅳ) is more bioavailable than Se(Ⅵ). Se accumulation caused oxidative stress in zebrafish tissues. Our findings represent a progress in research on the toxicological mechanism of Se exposure in fish and other aquatic organisms from the aspects of the antioxidant defense system. |
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