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| Determination of fludioxonil residues in tomato and soil by ultra-performance liquid chromatographytandem mass spectrometry |
| Received:September 23, 2018 |
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| KeyWord:fludioxonil;tomato;soil;residue;ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) |
| Author Name | Affiliation | E-mail | | ZHANG Yi-ting | College of Life Science and Technology, Harbin Normal University, Harbin 150025, China | | | JING Le-gang | College of Life Science and Technology, Harbin Normal University, Harbin 150025, China | jlgachxy@126.com | | ZHANG Xiao-bo | Safety and Quality Institute of Agricultural Products, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China | | | DONG Jian-nan | Safety and Quality Institute of Agricultural Products, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China | | | LIU Feng | Safety and Quality Institute of Agricultural Products, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China | | | CHEN Guo-feng | Safety and Quality Institute of Agricultural Products, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China | |
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| Abstract: |
| An ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method was established for the determination of fludioxonil residues in tomato and soil samples. The samples were extracted with aqueous acetonitrile, and then purified by primary secondary amine(PSA) and Florisil. Chromatographic separation was performed on an ACQUITY UPLC BEH C18(2.1 mm×50 mm, 1.7 μm) with a mobile phase composed of aqueous solution(containing 0.1% formic acid) and acetonitrile in gradient elution. Mass spectrometric detection was conducted in negative electrospray ionization(ESI) with multiple reaction monitoring(MRM) mode. The quantification of fludioxonil was performed using the external standard method. An excellent linear relationship between peak area(y) and mass concentration(x) of fludioxonil was obtained in the range of 0.01~2.00 mg·L-1. The linear equation was y=3 412.6x+163.95, and the correlation coefficient(r2) was 0.999 5. At three fortification levels of 0.01, 0.10 mg·kg-1 and 2.00 mg·kg-1, the average recovery rates of fludioxonil in the tomato and soil samples were in the ranges of 95.96%~103.17% and 88.21%~106.88%, with the relative standard deviations (RSD) of 1.8%~4.6% and 1.1%~6.2%, respectively. The limit of detection(LOD) for both the tomato and soil samples was 0.4 μg·kg-1. The limits of quantification(LOQ) for them were 1.4 μg·kg-1 and 1.3 μg·kg-1, respectively. This method is simple, rapid, accurate and sensitive, and is suitable for the analysis of fludioxonil residues in tomato and soil. |
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