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| Isolation, identification, and degradation characteristics of endophytic cypermethrin-degrading bacteria |
| Received:July 08, 2019 |
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| KeyWord:endophytic bacteria;cypermethrin;biodegradation;isolation and identification |
| Author Name | Affiliation | E-mail | | SUN Li-na | Institute of Ecological Environment Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Shanghai Engineering Research Center of Low-carbon Agriculture, Shanghai 201403, China | | | HUANG Kai-hua | Institute of Ecological Environment Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Shanghai Engineering Research Center of Low-carbon Agriculture, Shanghai 201403, China | | | GAO Xin-hua | Institute of Ecological Environment Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Shanghai Engineering Research Center of Low-carbon Agriculture, Shanghai 201403, China | | | CHEN Wei | Institute of Ecological Environment Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Shanghai Engineering Research Center of Low-carbon Agriculture, Shanghai 201403, China | | | BAI Na-ling | Institute of Ecological Environment Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Shanghai Engineering Research Center of Low-carbon Agriculture, Shanghai 201403, China | | | Lü Wei-guang | Institute of Ecological Environment Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Shanghai Engineering Research Center of Low-carbon Agriculture, Shanghai 201403, China | lwei1217@sina.com |
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| Abstract: |
| A cypermethrin-degrading endophytic bacteria was isolated from an insecticide factory in Shanghai, China. This isolate, designated as A-24, was identified as Achromobacter sp. based on morphological, physiological, biochemical characterization, and phylogenetic analysis of the 16S rRNA gene sequence. The strain could degrade 91.8% of 20 mg·L-1 cypermethrin within 48 h, and completely degrade 20 mg·L-1 cypermethrin within 72 h. Strain A-24 could also utilize cypermethrin as the sole carbon source for growth. The optimum temperature and pH for cypermethrin degradation by strain A-24 were 30℃ and 7.0, respectively. When the inoculation volume was more than 2% and the cypermethrin concentration was less than 50 mg·L-1, strain A-24 could rapidly degrade cypermethrin. High-performance liquid chromatography analysis indicated that the strain degraded cypermethrin via hydrolysis of the carboxylester linkage to yield 3-phenoxybenzaldehyde, which was further converted into 3-phenoxybenzoic acid under the catalysis of a dehydrogenase. This study provides a new strategy for removal of cypermethrin residues from plants by endophytic bacteria. |
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