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| Ecological effects of two earthworms on the enzymic activity and bacterial community structure in tetracycline-contaminated soil |
| Received:July 23, 2019 |
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| KeyWord:soil;earthworm;tetracycline;enzyme activity |
| Author Name | Affiliation | E-mail | | LUO Shu-wen | Guangdong Ocean University, Zhanjiang 524088, China | | | ZHEN Zhen | Guangdong Ocean University, Zhanjiang 524088, China | | | LI Wen-qing | Guangdong Ocean University, Zhanjiang 524088, China | | | ZHANG Wei-jian | Guangdong Ocean University, Zhanjiang 524088, China | | | WU Wei-jian | Guangdong Ocean University, Zhanjiang 524088, China | | | REN Lei | Guangdong Ocean University, Zhanjiang 524088, China | | | LIN Zhong | Guangdong Ocean University, Zhanjiang 524088, China | linzhong18@163.com |
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| Abstract: |
| Soil enzymes and microorganisms can catalyze biochemical reactions in the soil, and are often selected as key indicators of the self-purification ability of the soil and soil fertility. Two earthworms(epigeic Eisenia foetida and endogeic Amynthas robustus)were selected to investigate their ecological effects on soil enzymic activities in tetracycline-contaminated soil. Redundancy analysis was used to determine which environmental factors were more influential on enzymic activity and high-throughput sequencing was used to detect bacterial community structure. The results showed that the activities of invertase(2.15~4.98 mg·g-1·d-1), urease(0.55~1.70 mg·g-1·d-1), catalase (0.54~1.08 mL·g-1·20 min-1), and dehydrogenase(0.78~5.14 μg·g-1·d-1)in earthworm treatments were significantly higher than that in the natural control(3.24 mg·g-1·d-1, 0.66 mg·g-1·d-1, 0.89 mL·g-1·20 min-1, and 2.03 μg·g-1·d-1, respectively)and the sterilized control (0.70 mg·g-1·d-1, 0.23 mg·g-1·d-1, 0.24 mL·g-1·20 min-1, and 0.21 μg·g-1·d-1, respectively). However, acid phosphatase activity in earthworm treatments were lower than that in the natural control(0.17 mg·g-1·d-1)and sterilized control(0.04 mg·g-1·d-1). Earthworms could stimulate Proteobacteria, Bacteroidetes, and Firmicutes, whereas they inhibited Chloroflexi, Actinobacteria, Acidobacteria, and Gemmatimonadetes at the phylum level. Redundancy analysis indicated that the two ecological earthworms enhanced the activity of invertase, urease, catalase, and dehydrogenase, and inhibited acid phosphatase activity primarily through influencing soil pH, organic matter, humic acid, and humin. Specifically, epigeic Eisenia foetida stimulated sucrase activity than endogeic Amynthas robustus, whereas Amynthas robustus enhanced urease and catalase activities. |
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