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| Analysis of the microbial community of pig manure fermented with straw litter in an ectopic fermentation system |
| Received:September 16, 2019 |
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| KeyWord:ectopic fermentation system;straw litter;microbial community |
| Author Name | Affiliation | E-mail | | YE Shi-hao | College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China | | | SHEN Qi | Institute of Plant Protection and Microbiology, Zhejiang Academy of Agriculture Science, Hangzhou 310021, China | | | LI Yuan-cheng | Institute of Plant Protection and Microbiology, Zhejiang Academy of Agriculture Science, Hangzhou 310021, China | | | SUN Hong | Institute of Plant Protection and Microbiology, Zhejiang Academy of Agriculture Science, Hangzhou 310021, China | | | YAO Xiao-hong | Institute of Plant Protection and Microbiology, Zhejiang Academy of Agriculture Science, Hangzhou 310021, China | | | WU Yi-fei | Institute of Plant Protection and Microbiology, Zhejiang Academy of Agriculture Science, Hangzhou 310021, China | | | WANG Xin | Institute of Plant Protection and Microbiology, Zhejiang Academy of Agriculture Science, Hangzhou 310021, China | | | LI Wei-lin | College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China | | | TANG Jiang-wu | Institute of Plant Protection and Microbiology, Zhejiang Academy of Agriculture Science, Hangzhou 310021, China | tangjiangwu@sina.com |
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| Abstract: |
| The objective of this study was to explore the effect of different straws on the fermentation process of pig manure in an ectopic fermentation system(EFS)and to reveal the succession of microbial communities on different straws. Three types of litter were used in this experiment. Group Y was composed of rapeseed straw, wood chips, and rice chaff; group S was composed of rice straw, wood chips, and rice chaff; and group CK was composed of wood chips and rice chaff. Litter samples were collected at different times during the EFS process to analyze the physicochemical properties and conduct Illumina high-throughput sequencing. The results showed that the microbial diversity in group CK was significantly lower than those of groups S and Y. The trend of microbial succession was similar in all vessels during the EFS process, but the degree of change varied considerably. The abundance of Proteobacteria in group CK decreased by 75.0%, while those in group S and group Y decreased by 38.0% and 23.2%, respectively. The dominant microorganisms in the thermophilic period of the three treatments were Bacillus, Lactobacillus, Sphingobacterium, and Pseudomonas, but their abundance varied. Gene prediction and functional analysis indicated that the abundances of lipid, amino acid, and carbohydrate metabolism-related genes in group Y were the highest. The changes in the dynamics of physicochemical indexes in all treatments were similar in the fermentation process, but the heating rate, overall temperature, and dissolved N concentration of group Y were the highest. The results suggested that the addition of straw to litter increased the microbial richness and diversity in the EFS. Meanwhile, rapeseed straw made the organic matter metabolism more active and contributed to high-temperature operation of the EFS and the formation of organic fertilizer. |
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