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| Effects of exogenous vanadium stress on vanadium accumulation and subcellular distribution, and non-protein thiol content in maize(Zea mays L.)crops |
| Received:January 15, 2020 |
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| KeyWord:vanadium;maize (Zea mays L.)seedling;accumulation;subcellular distribution;non-protein thiol compound |
| Author Name | Affiliation | | HOU Ming | College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China | | HUO Yan | College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China | | ZHANG Zhi-zhuan | College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China | | WEI Ming-feng | College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China |
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| Abstract: |
| The purpose of the present study is to investigate vanadium (V)-tolerance and-enrichment mechanisms in maize (Zea mays L.) crops. Maize seedlings were treated with varying concentrations of V (0, 100, 200, 300, and 500 mg·kg-1)to investigate the differences in bioaccumulation and subcellular distribution of V and the responses of non-protein thiols (NPTs)to V exposure in maize seedlings by differential centrifugation and 5,5'-dithio bis-(2-nitrobenzoic acid)colorimetry, respectively. The findings revealed that V bioaccumulation in different parts of maize plants decreased in the following order:roots > leaves > stems > grains; 74.8%~95.6% of V was accumulated in the roots. With an increase in V concentration, the bioconcentration factor increased from 0.061 to 0.306, whereas the translocation factor decreased from 0.336 to 0.108, thereby reducing the toxicity of excessive V to the stems and leaves of maize seedlings. V was mainly accumulated in the cell wall (F1)and soluble component (F4)of plant, both of which account for 60.76% to 75.75% of the total accumulation in the shoots, and from 82.66% to 87.02% of the total accumulation in the roots. V at certain concentrations in soil (≤ 300 mg·kg-1)could promote the synthesis of NPTs and phytochelatins (PCs); plants utilize glutathione to synthesize PCs, thereby reduce the toxicity of V. Thus, the strategies employed by maize seedlings against V toxicity involve cell wall deposition, heavy metal compartmentalization, and thiol compound synthesis. |
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