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| Isolation and screening of deodorant bacterial strains from chicken manure and analysis of their deodorant effects |
| Received:December 09, 2019 |
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| KeyWord:chicken manure;deodorant microorganisms;isolation and screening;deodorizing effect;complex microbial system |
| Author Name | Affiliation | E-mail | | LI Yue | College of Resources, Sichuan Agricultural University, Chengdu 611130, China | | | LI Cheng-cheng | College of Resource and Environment, China Agricultural University, Beijing 100094, China | | | LI Jing | College of Resources, Sichuan Agricultural University, Chengdu 611130, China | | | ZHANG Xiao-yue | College of Resources, Sichuan Agricultural University, Chengdu 611130, China | | | ZHANG Zi-wei | College of Resources, Sichuan Agricultural University, Chengdu 611130, China | | | HUANG Yi-man | Wangcang County Agricultural and Rural Bureau, Guangyuan 628200, China | | | LIU Hao | Modern Agricultural Development Promotion Center, Luzhou City Agricultural and Rural Bureau, Luzhou 646000, China | | | LUO Hong | College of Resources, Sichuan Agricultural University, Chengdu 611130, China | | | ZHANG Xiao-ping | College of Resources, Sichuan Agricultural University, Chengdu 611130, China | | | ZHAO Ke | College of Resources, Sichuan Agricultural University, Chengdu 611130, China | zhaoke82@126.com |
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| Abstract: |
| In order to discover strains possessing deodorant effects, which can be used in the harmless treatment of chicken manure, deodorizing bacteria were isolated from chicken manure, and their complexes were constructed. Deodorizing bacteria were screened using several methods:enrichment culture, a plate dilution method, gas production test, ammonia removal test, hydrogen sulfide removal test, and cellulose degradation test. Isolates were then identified by 16S rRNA gene sequencing. Finally, the complex microbial systems were constructed, based on the results of the antagonism effect among strains. Five strains possessing deodorizing functions were isolated; these were Bacillus sp. (MS03), Bacillus velezensis (MS07), Brevibacterium frigoritolerans (MS11), Staphylococcus xylosus (MS42)and Corynebacterium variabile (MS82). The results show that the deodorizing efficiencies of complexes C2 (MS03+MS07)and C4 (MS03+MS82)were higher than 60%, and significantly higher than that for any single bacteria, or for other complexes. Our results indicate that complexes C2 and C4 both have great potential for the harmless treatment of chicken manure. |
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