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| Metabolic enzymes and DEHP metabolic dynamics in rats under short-term exposure to DEHP |
| Received:October 18, 2020 |
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| KeyWord:di-(2-ethylhexyl) phthalate (DEHP);oxidative stress;phase Ⅱ enzymes;metabolism |
| Author Name | Affiliation | E-mail | | HUANG Zhuo-quan | SCAU(Chaozhou) Food Institute Co. Ltd., Chaozhou 521000, China
College of Food Sciences, South China Agricultural University, Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou 510642, China | | | LIU Rui-jing | College of Food Sciences, South China Agricultural University, Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou 510642, China | | | HUANG Shao-wen | College of Food Sciences, South China Agricultural University, Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou 510642, China | | | HE Yong-jian | College of Food Sciences, South China Agricultural University, Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou 510642, China | | | LIU Huan | College of Food Sciences, South China Agricultural University, Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou 510642, China | | | ZHENG Dong-dong | College of Food Sciences, South China Agricultural University, Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou 510642, China | | | LIU Chun-hong | SCAU(Chaozhou) Food Institute Co. Ltd., Chaozhou 521000, China
College of Food Sciences, South China Agricultural University, Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou 510642, China | liuch@scau.edu.cn |
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| Abstract: |
| To investigate the metabolic changes in di-(2-ethylhexyl) phthalate(DEHP) in rats and the effects of DEHP on the oxidase and phase Ⅱ enzymes after repeated short-term exposure to DEHP, 32 specific pathogen-free(SPF) grade male Sprague Dawley(SD) rats were randomly assigned to four groups(n=8 each), including normal control and DEHP low(300 mg·kg-1), medium(1 000 mg·kg-1), and high dose(3 000 mg·kg-1) groups. All rats were treated via gastric gavage for 28 days. Oxidation indicators in the serum and liver, such as total superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), malondialdehyde(MDA), and phase Ⅱ enzymes in the liver, such as UDP-glucuronosyltransferase 1(UGT1), glutathione S-transferase(GST), and glutathione S-transferase-pi(GST-pi), were detected with ELISA. The levels of mono-(2-ethylhexyl) phthalate(MEHP, a metabolite of DEHP) in the serum and urine were determined using highperformance liquid chromatography(HPLC). The results indicated that DEHP exposure reduced the activities of SOD and GSH-Px and increased the level of MDA and the contents of phase Ⅱ enzymes UGT1, GST, and GST-pi in the rat liver significantly. During the 28 days repeated exposure, MEHP concentrations initially increased and then decreased, and those of each treatment reached the highest value on the 7th day of DEHP exposure. The results showed that DEHP caused oxidative damage to rat livers and induced the expression of phase Ⅱ enzymes. The changes in MEHP in urine were positively correlated with the DEHP dosages, and the highest values were found on the 7th day of exposure. |
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