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| Screening and composition of the microbial consortium with corn straw decomposition under low temperature |
| Received:January 14, 2021 |
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| KeyWord:low temperature;corn straw;microbial consortium;degradation characteristics;microbial composition |
| Author Name | Affiliation | E-mail | | ZHANG Xin | Agricultural College, Inner Mongolia Agricultural University, Hohhot 010019, China | | | Qinggeer | Agricultural College, Inner Mongolia Agricultural University, Hohhot 010019, China
Key Laboratory of Crop Cultivation and Genetic Improvement in Inner Mongolia Autonomous Region, Hohhot 010019, China | | | GAO Julin | Agricultural College, Inner Mongolia Agricultural University, Hohhot 010019, China
Key Laboratory of Crop Cultivation and Genetic Improvement in Inner Mongolia Autonomous Region, Hohhot 010019, China | nmgaojulin@163.com | | YU Xiaofang | Agricultural College, Inner Mongolia Agricultural University, Hohhot 010019, China
Key Laboratory of Crop Cultivation and Genetic Improvement in Inner Mongolia Autonomous Region, Hohhot 010019, China | yuxiaofang75@163.com | | HU Shuping | Key Laboratory of Crop Cultivation and Genetic Improvement in Inner Mongolia Autonomous Region, Hohhot 010019, China
Vocational and Technical College, Inner Mongolia Agricultural University, Baotou 014100, China | | | ZHANG Bizhou | Agricultural College, Inner Mongolia Agricultural University, Hohhot 010019, China | | | HAN Shengcai | Key Laboratory of Crop Cultivation and Genetic Improvement in Inner Mongolia Autonomous Region, Hohhot 010019, China
Horticulture and Plant Protection College, Inner Mongolia Agricultural University, Hohhot 010019, China | | | FENG Biao | Agricultural College, Inner Mongolia Agricultural University, Hohhot 010019, China | |
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| Abstract: |
| To promote in-situ corn straw decomposition in cold northern regions, this study screened the microbial consortium with corn straw degradation under low temperatures. Fifty-seven bacterial source samples from different areas of Inner Mongolia were selected as screening objects by enrichment culture in a low temperature（15 ℃）subculture with corn straw as the carbon source, and using the straw degradation rate and enzyme activity of different cultures as screening indexes. The compound strains with high efficiency in degrading corn straw were selected. The degradation characteristics and microbial community diversity of complex strains were determined. The results showed that three complex strains, numbered M44, M14, and M2, with corn straw degradation under low temperature were screened from the 57 materials using the multi-level progressive method. The corn straw degradation rates of M44, M14, and M2 were 35.33%, 33.34%, and 31.33% at 15 ℃ for 20 d, respectively. Among the corn straw degradation rates, that of M44 was 12.25% higher than that of the control （GF- 20）; the hemicellulose and lignin degradation rates of straw were 5.47% and 23.13% higher than those of the control, respectively. The activities of filter paper enzyme, endoglucanase, xylanase, and laccase were 7.41%, 1.44%, 13.85%, and 17.88% higher than those of the control, respectively. High-throughput sequencing revealed that Proteobacteria, Firmicutes, and Bacteroidetes were the dominant phyla in all consortia. M44 and M14 were predominantly composed of Trichococcus, Acinetobacte, Azospirillum, and Enterococcus. M2 was mainly composed of Delftia and Sphingobacterium. From this analysis, it is conclude that the compound strain M44 obtained using corn straw as a carbon source can effectively degrade corn stalks at low temperatures （15 ℃）. |
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