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| Pilot-scale study on effects of exogenous additives on reducing NH3 and H2S emissions from pig manure compost |
| Received:November 06, 2020 |
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| KeyWord:antibiotic resistance gene;relative abundance;livestock and poultry manure;treatment process;removal efficiency |
| Author Name | Affiliation | E-mail | | YAN Lei | College of Resources and Environment Northeast Agricultural University, Harbin 150036, China | | | DING Gongyao | College of Resources and Environment Northeast Agricultural University, Harbin 150036, China | | | YANG Fengxia | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | RUAN Rong | College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China | | | HAN Bingjun | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | ZHI Suli | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | zhisuli87@163.com | | ZHANG Keqiang | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | keqiangzhang68@163.com |
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| Abstract: |
| In order to investigate the removal efficiency of antibiotic resistance gene(ARGs) from livestock and poultry manure by treatment processes, different livestock farms in Tianjin City that have typical treatment processes were selected to detect of 13 ARGs (comprising 5 types) and intI1 in feces and sewage using real-time fluorescence based quantitative PCR. The results revealed that the target ARGs and intI1 were highly prevalent in feces and sewage(10-7~10-1). Among these, the relative abundance of quinolone ARGs was lowest(10-7~10-5) in feces on the pig, chicken and cattle farms, and the levels of tetracycline and sulfonamide ARGs contamination were relatively higher, with relative abundances of 10-4~10-1 and 10-4~10-2, respectively. The relative abundances of the 13 ARGs and intI1 in pig farm sewage(10-3~10-2) were generally higher than those in cattle farm sewage(10-6~10-4). For ARGs in sewage, the treatment processes on pig and cattle farms could remove most ARGs, particularly tetracycline ARGs, with relative abundance(lgs) reduced by 0~2.09. In addition, the anaerobic fermentation and storage units used for pig farm wastewater treatment processes showed better efficiency for the removal of ARGs, whereas adjustment units were more efficient in the removal of ARGs on cattle farms. With respect to livestock and poultry feces, the ectopic fermentation bed, composting, and aseptic drying treatments could remove most of the target ARGs. Treatment of pig manure by ectopic fermentation beds could reduce the relative abundance(lgs) of 10 ARGs, with an average reduction of 1.11, whereas after the composting of cattle and chicken manure, the relative abundance(lgs) of 8 and 9 ARGs was reduced by 0.83 and 1.32, respectively. Furthermore, after sterilization and drying, the relative abundances(lgs) of 5 ARGs in cattle manure was reduced by 1.12 on average. However, it is worth noting that the levels of sulfonamide ARGs showed an increasing trend after these treatment processes, and therefore future studies should focus on the contamination caused by sulfonamide resistance genes. |
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