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| Effects of fomesafen and its degrading bacteria on soybean growth and biological nitrogen fixation |
| Received:March 05, 2021 |
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| KeyWord:fomesafen;soybean;rhizobium;biological nitrogen fixation;ammonia oxidizing archaea(AOA);ammonia oxidizing bacteria (AOB) |
| Author Name | Affiliation | E-mail | | ZHOU Cong | Co-innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China | | | CHEN Wei | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China | | | GAO Yan | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China | ygao@jaas.ac.cn | | SHI Man | College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311100, China | | | LI Jiangye | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China | | | LIU Lizhu | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China | | | CHEN Jinlin | Co-innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China | jlchen@njfu.edu.cn |
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| Abstract: |
| A fomesafen-degrading bacteria strain, Sinorhizobium sp. W16, was isolated and screened from root nodules of soybean grown in soil contaminated with fomesafen; however, whether inoculation of this strain could improve soybean growth and the biological nitrogen fixation capacity in plant-soil system remains unclear. A pot experiment was carried out to investigate the effects of Sinorhizobium sp. W16 inoculation on soybean(Su C1008) growth, nitrogen content, nodule nitrogenase activity, and nitrogen cycle-related functional genes abundance in the rhizosphere soil. The results showed that the use of fomesafen(calculated by available ingredient) over 450 g·hm-2 significantly reduced soybean biomass, inhibited the activities of nitrogen fixing enzymes in soybean nodules and urease in rhizospheresoil, and decreased the gene abundance of nitrogen-fixing bacteria(nifH), ammonia oxidizing archaea(AOA), ammonia oxidizing bacteria (AOB) in the rhizosphere-soil, and thereby limited the biological nitrogen fixation and organic nitrogen transformation in plantrhizosphere system. Inoculation of the Sinorhizobium sp. W16 strain significantly improved the degradation rate of fomesafen by up to 81.97%, and enhanced the dry weight of soybean nodules, activities of nodule nitrogenase and gene abundances of soil nifH, resulting in the increase of nitrogen fixation in soybeans-rhizosphere system. Meanwhile, the strain inoculation stimulated urease activity and enhanced the abundance of soil AOA and AOB, leading to the increase of the available nitrogen in the rhizosphere-soil. Combined, these effects of Sinorhizobium application led to the increase in nitrogen content of soybean plants by 15.85%~24.93%.The use of fomesafen significantly inhibited the nitrogen fixation in soybeans-rhizosphere system; however inoculation of the Sinorhizobium sp. W16 strain could not only degrade the fomesafen in soil effectively, alleviating the totoxicity of fomesafen to soybeans, but could also enhance the biological nitrogen fixation and available nitrogen in the soybeans-rhizosphere system, and nitrogen accumulation by soybeans, implying a good prospect for remediating fomesafen-contaminated soil and enhancing soybean nitrogen fixation. |
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