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| Effects of methomyl on antioxidant defense system of zebrafish embryos |
| Received:June 20, 2021 |
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| KeyWord:methomyl;zebrafish;embryo;antioxidant defense factor;oxidative damage |
| Author Name | Affiliation | E-mail | | LU Yan | Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China | | | CHEN Xi | Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Environmental Factors(Wuxi), Ministry of Agriculture and Rural Affairs/Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | QIU Liping | Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Environmental Factors(Wuxi), Ministry of Agriculture and Rural Affairs/Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | HU Gengdong | Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Environmental Factors(Wuxi), Ministry of Agriculture and Rural Affairs/Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | SONG Chao | Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Environmental Factors(Wuxi), Ministry of Agriculture and Rural Affairs/Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | FAN Limin | Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Environmental Factors(Wuxi), Ministry of Agriculture and Rural Affairs/Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | ZHENG Yao | Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Environmental Factors(Wuxi), Ministry of Agriculture and Rural Affairs/Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | MENG Shunlong | Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Environmental Factors(Wuxi), Ministry of Agriculture and Rural Affairs/Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, Wuxi 214081, China | mengsl@ffrc.cn | | CHEN Jiazhang | Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Environmental Factors(Wuxi), Ministry of Agriculture and Rural Affairs/Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, Wuxi 214081, China | chenjz@ffrc.cn |
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| Abstract: |
| To explore the effects of methomyl on the antioxidant defense system and oxidative damage in zebrafish embryo development, zebrafish embryos were exposed to methomyl solutions of 0, 2, 20 μg·L-1, and 200 μg·L-1 concentrations within 6 h after fertilization. The contents and activities of superoxide dismutase(SOD), catalase(CAT), glutathione reductase(GR), reduced glutathione(GSH), oxidized glutathione(GSSG), and malondialdehyde(MDA) were measured at 24, 48, 72 h, and 96 h, respectively. After exposure to methomyl, the SOD activity decreased at first, then increased and decreased, with significant differences between each concentration group and the control group(P<0.05). The CAT activity was first induced and then inhibited; the CAT activity in 20 μg·L-1 and 200 μg·L-1 treatment groups was significantly higher than that in the control group(P<0.05). The GR, GSH, and GSSG activity increased at first and then decreased. Compared with the control group, the MDA content in zebrafish embryos in each concentration group increased, first increasing, then decreasing, and finally increasing again. Methomyl has an oxidative stress effect on zebrafish embryos, changing the activity level of antioxidant defense factors and MDA content in zebrafish embryos by inhibiting or promoting it, which interferes with the normal operation of the antioxidant defense system, with oxidative damage effects. |
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