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| Copper accumulation patterns in different tissues of juvenile Anodonta woodiana individuals |
| Received:September 01, 2021 |
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| KeyWord:Anodonta woodiana;copper;bioaccumulation;target tissue;exposure time;exposure concentration;exposure concentration |
| Author Name | Affiliation | E-mail | | LIU Kai | Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China | | | CHEN Xiubao | Key Laboratory of Fishery Ecological Environment Assessment and Research Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | LIU Hongbo | Key Laboratory of Fishery Ecological Environment Assessment and Research Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | JIANG Tao | Key Laboratory of Fishery Ecological Environment Assessment and Research Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | YANG Jian | Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China
Key Laboratory of Fishery Ecological Environment Assessment and Research Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | jiany@ffrc.cn |
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| Abstract: |
| This study aimed to investigate the tissue-specific toxic effects of copper（Cu）on the freshwater mussel Anodonta woodiana at the juvenile stage, when this species is more sensitive to heavy metal toxicity. Five different Cu concentrations（2.0, 1.0, 0.1, 0.01 mg·L-1, and 0.005 mg·L-1）were set, based on the results of a preliminary 96 h-EC50 experiment and the China Water Quality Standard for Fisheries （GB 11607—1989）. The mussels were exposed to Cu for 24, 48, 72 h, and 96 h, and tissue Cu content were assayed by inductively coupled plasma mass spectrometry（ICP-MS）. Such analysis showed that Cu content in the exposed groups were significantly higher than those in the control groups. In particular, Cu content in the whole soft tissue increased with increasing exposure Cu concentrations and longer exposure times. The highest tissue-specific Cu content was observed in the mantle, with a value of 669.2 μg·g-1. The gill and mantle were the most sensitive tissues, since they displayed significantly increased Cu content already after 24 h of exposure, and reached Cu levels that were significantly higher than those in the foot and digestive glands. Cu content in gill and mantle tissues reached a maximum at 96 h and 72 h, respectively. Nevertheless, the content of Cu at 96 h was highest in the digestive glands and axe-shaped foot. Therefore, these tissues are potential targets for Cu accumulation. This study provides a theoretical basis for the characterization of Cu accumulation in tissues of A. woodiana, the bioindicator species of the“Freshwater Mussel Watch”project, as well as technical support for monitoring and evaluating Cu pollution in freshwater environments. |
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