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| Bioreduction of Cr（Ⅵ）by Bacillus sp. T124 |
| Received:September 09, 2021 |
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| KeyWord:chromium;Bacillus;bioreduction;coexisting ions;environmental remediation |
| Author Name | Affiliation | E-mail | | JIANG Yi | School of Environmental and Safety Engineering of Changzhou University, Changzhou 213164, China | | | TANG Ding | School of Environmental and Safety Engineering of Changzhou University, Changzhou 213164, China | | | ZHOU Yucheng | School of Environmental and Safety Engineering of Changzhou University, Changzhou 213164, China | | | DING Congcong | School of Environmental and Safety Engineering of Changzhou University, Changzhou 213164, China | | | ZHAO Xingqing | School of Environmental and Safety Engineering of Changzhou University, Changzhou 213164, China | zhaoxq@cczu.edu.cn | | CHAI Yuhong | School of Environmental and Safety Engineering of Changzhou University, Changzhou 213164, China
Huaide College of Changzhou University, Jingjiang 214500, China | |
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| Abstract: |
| To better understand the mechanism of reducing Cr（Ⅵ）, A Cr tolerant strain Bacillus sp. T124 from a heavy metal polluted soil around a mining area was screened; the efficiency of removing Cr（Ⅵ）, and the reducing products of the strain were studied. Results showed that Bacillus sp. T124 could effectively remove Cr（Ⅵ）, and the reduction rate was 43.2% in LB medium with an initial concentration of 100 mg·L-1 of Cr（Ⅵ）for 48 h. In addition, among the bacterial electron donor for Cr（Ⅵ）reduction, fructose was the most effective of the six carbon sources（glucose, fructose, sucrose, lactose, sodium acetate, and mannitol）Cr（Ⅵ）, and the addition of HCO3- had the most obvious inhibitory effect on the biological reduction of Cr（Ⅵ）. Scanning electron microscope（SEM）results showed no significant changes in the morphology of the cells treated with Cr（Ⅵ）. Diffraction of X-rays（XRD）and Raman spectra revealed that the reduction product of Cr（Ⅵ） was Cr2O3. Fourier transform infrared spectrometer（FT-IR）results showed that alkyl and carboxyl groups and polysaccharides were involved in the reduction process, and X-ray photoelectron spectroscopy（XPS）analysis suggested that the way to remove Cr（Ⅵ）was bioreduction rather than biosorption. These results showed that bioreduction was the main process that Bacillus sp. T124 use in removing Cr （Ⅵ）, and carbon source and coexisting ions were the key factors involved in the process of Cr（Ⅵ）removal by Bacillus sp. T124. |
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