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| Toxicity and metabolism of adult zebrafish brain tissue induced by combined exposure to graphene oxide and polycyclic aromatic hydrocarbons |
| Received:October 27, 2021 |
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| KeyWord:graphene oxide;polycyclic aromatic hydrocarbons;zebrafish;compound exposure;metabolomes |
| Author Name | Affiliation | E-mail | | SUN Jing | Center of Eco-environmental Monitoring and Scientific Research, Administration of Ecology and Environment of Haihe River Basin and Beihai Sea Area, Ministry of Ecology and Environment, Tianjin 300061, China
Key Laboratory of Pollution Processes and Environmental Criteria(Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China | | | LI Wei | Center of Eco-environmental Monitoring and Scientific Research, Administration of Ecology and Environment of Haihe River Basin and Beihai Sea Area, Ministry of Ecology and Environment, Tianjin 300061, China | | | CONG Rui | Center of Eco-environmental Monitoring and Scientific Research, Administration of Ecology and Environment of Haihe River Basin and Beihai Sea Area, Ministry of Ecology and Environment, Tianjin 300061, China | | | HU Xiangang | Key Laboratory of Pollution Processes and Environmental Criteria(Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China | | | OUYANG Shaohu | Key Laboratory of Pollution Processes and Environmental Criteria(Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China | ouyangshaohu@nankai.edu.cn |
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| Abstract: |
| With the increase of application and research on graphene oxide (GO), the risk possibility of its release into the environment has increased, where it may interact with polycyclic aromatic hydrocarbons (PAHs). However, the effects of GO and PAH co-exposure on aquatic ecotoxicity and related metabolic mechanisms are still largely unknown. In this work, adult zebrafish was chosen as a model organism to investigate the toxic effects of single exposure to different concentrations of GO (0.01 mg · L-1 and 0.1 mg · L-1) and PAHs (including 16 kinds of optimal control PAHs, each with a concentration of 5 μg · L-1), and combined exposure to GO-PAHs at the corresponding concentration for 21 days on adult zebrafish brain tissues and the metabolism in these tissues. Enzyme results showed that 0.1 mg·L-1 GO and 0.1 mg·L-1 GO-PAHs could significantly (P<0.05) decrease aromatic hydrocarbon receptor activity, and that all the treatments (except for PAHs) decreased concentration of cytochrome P4501A1 in adult zebrafish brain tissue. Metabolomics analysis results showed that 0.1 mg·L-1 GO, and 0.1 mg·L-1 GO-PAHs exposure groups significantly influenced amino acids and fatty acids levels in adult zebrafish brain tissue. Moreover, when compared with the GO and PAHs exposure groups, the enzyme response and metabolite levels induced by the GO-PAHS combined exposure were found to be close to those induced by the GO treatment, revealing that GO was dominant in inducing metabolic toxicity in brain tissue during combined exposure of GO and PAHs in adult zebrafish. |
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