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| Influences of rape straw and microbial agent incorporation on facility cultivation watermelon soil fertility and the composition and function of microbes under anoxic conditions |
| Received:January 13, 2022 |
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| KeyWord:facility cultivation;soil improvement;rape straw;microbial composition;soil function |
| Author Name | Affiliation | E-mail | | WANG Qingfeng | Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China | | | CHU Changbin | Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China | | | ZHAO Zheng | Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China | | | YANG Qiangang | Hangzhou Jinjiang Group Co. Ltd., Hangzhou 310005, China | | | DONG Yuexia | Shanghai Agricultural and Technology Service Center, Shanghai 200335, China | | | ZHOU Deping | Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China | zhoudeping@saas.sh.cn | | WU Shuhang | Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China | wushuhang88@163.com |
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| Abstract: |
| This paper aims to investigate the influence of rape straw and microbial agent application on soil properties, and effects on microbial composition and functional traits after long-term watermelon continuous cultivation, to provide the theoretical basis for improving soil quality after extensive agricultural intensification using rape straw. Based on a continuous greenhouse watermelon planting experiment, the study conducted a pot experiment with pre-flooding(CK), pre-flooding and fresh rape straw addition(GSL), and GSL plus microbial agents(including Aspergillus niger and Bacillus velezensis, which can promote the decomposition of plant straw, B_GSL). This study tracked the impact of those treatments on the microbial diversity and composition by using Illumina Miseq. A functional annotation of prokaryotic taxa analysis was used to reveal the functional changes under the addition of rape straw and other treatments. The results showed that soil organic matter and pH were significantly increased, which pH increased from 5.93 to 6.81 and 6.57 in GSL and B_GSL treatments, respectively. The addition of rape straw(including GSL and B_GSL treatments)had no significant effect on microbial Shannon and Shannoneven diversity indices, but significantly increased richness index(Chao1 index and ACE index). Compared to CK treatments, Chao1 index in GSL and B_GSL treatments were increased by 8.7% and 6.7%, respectively. Soil microbial composition was significantly changed by rape straw addition(including GSL and B_GSL), but there was more similarity between GSL and B_GSL according to principal coordinates analysis(PCoA). The Student's T test showed that GSL and B_GSL significantly increased nitrate respiration and associated functional traits compared to CK, while B_GSL also significantly promoted methanol oxidation, and denitrification function. In-addition, B_GSL significantly decreased aerobic ammonia oxidation and nitrification functions compared to CK and GSL treatments, indicating that the addition of microbial agents promoted the functional application of rape straw. This research demonstrated that the addition of rape straw (including GSL and B_GSL treatments)improved soil fertility, increased soil microbial richness, changed soil microbial community composition, and functional traits. The results also suggest that the addition of microbial agents and rape straw would promote the functional application of rape. |
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