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| Advances in the detection technology of pathogenic microorganisms in soil |
| Received:October 11, 2022 Revised:November 03, 2022 |
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| KeyWord:biosafety and biosecurity;soil pathogenic microorganism;rapid detection technique;molecular biology tool;Raman spectroscopy |
| Author Name | Affiliation | E-mail | | XU Fei | Fujian Key Laboratory of Watershed Ecology, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China
University of Chinese Academy of Sciences, Beijing 100049, China
College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China | | | YANG Kai | Fujian Key Laboratory of Watershed Ecology, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China | | | ZHU Longji | Fujian Key Laboratory of Watershed Ecology, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China | | | LIN Wenfang | Fujian Key Laboratory of Watershed Ecology, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China | | | CUI Li | Fujian Key Laboratory of Watershed Ecology, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China | lcui@iue.ac.cn |
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| Abstract: |
| Pathogenic microorganisms（e. g., bacteria, viruses, and fungi）are ubiquitous in soil environments, threatening global public health. The development of a rapid detection technology for environmental pathogens is of great significance to reduce the spread of pathogens, prevent and control infectious diseases, and maintain biosecurity. This article reviewed the recently developed and state-of-theart techniques for detecting pathogenic microorganisms, including chromogenic cultivation, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, molecular biology tools, Raman spectroscopy, flow cytometry, and biosensors. A comprehensive overview and comparison of the principles, applications, advantages, and disadvantages of the presented techniques were shown. Finally, we put forward important perspectives for the future development of rapid detection methods, aiming to promote rapid diagnosis of soil pathogenic microorganisms and provide guidance for the control of biological risks. |
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