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| Preparation of biochar immobilized SM3 bacterial agent and degradation of triadimefon |
| Received:November 11, 2022 Revised:December 06, 2022 |
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| KeyWord:biochar;triadimefon;microbial immobilization technology;biodegradation |
| Author Name | Affiliation | E-mail | | WANG Ying | School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | LIN Fengxiang | School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | YUE Xingyu | School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | ZHANG Sai | School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | WU Zongbin | School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | SHI Yanhong | School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | shiyh@ahau.edu.cn |
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| Abstract: |
| To address the problem of triadimefon contamination in the environment, this study prepared an immobilized bacterial agent by adsorption method using biochar as a carrier and an efficient triazolone degrading bacterium Stenotrophomonas maltophilia(SM3)as an immobilized strain. The immobilized carriers with characteristics of large specific surface area and good adsorption performance were preferably selected from four types of biochar, and their preparation conditions were optimized, based on which the stability of biochar immobilized bacterial agents and their degradation effects on triazolone were evaluated. The results showed that among these four, Camellia oleifera biochar had large specific surface area and pore space, rich functional groups, and better degradation effect of immobilized bacterial agent on triadimefon than the other three, and hence was selected as the carrier for immobilization of SM3 bacteria. The singlefactor optimization test showed that the degradation rate of triadimefon by the immobilized bacterial agent reached 93.27% when prepared at a carrier dosage of 20 mg · mLP＞-1P＞, SM3 inoculum of 5%, immobilization temperature of 30℃, and immobilization time of 36 h. The degradation rate of the immobilized bacterial agent prepared under the optimal conditions was reported reaching 81.73% and 58.18% for triadimefon after 28 d at 4℃, and at room temperatures(25-35℃), with the bacterial agent displaying good stability. The preparation of immobilized SM3 bacterial agent from Camellia oleifera biochar thus provides a good bioremediation material to reduce triadimefon pollution in the environment. |
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