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| Isolation and degradation characteristics of deoxynivalenol degrading strain A4 |
| Received:November 08, 2022 Revised:December 06, 2022 |
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| KeyWord:deoxynivalenol;Devosia sp.;degradation characteristic;genomic difference analysis |
| Author Name | Affiliation | E-mail | | YU Yangguang | School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210014, China | | | HU Junqiang | Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210014, China
College of Life Science, Nanjing Agricultural University, Nanjing 210095, China | | | WANG Gang | School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210014, China | | | GAO Hongxia | Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210014, China | | | QIU Han | Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210014, China
College of Life Science, Nanjing Agricultural University, Nanjing 210095, China | | | ZHANG Yuhang | Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210014, China | | | LIU Xin | School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210014, China | | | SHI Jianrong | School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210014, China | | | XU Jianhong | School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210014, China
College of Life Science, Nanjing Agricultural University, Nanjing 210095, China | xujianhongnj@126.com |
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| Abstract: |
| The purpose of this study was to isolate deoxynivalenol(DON)degrading strain and to evaluate its degradation characteristics and mechanism, so as to provide strain resources for the detoxification of DON-contaminated grains and feeds. Soil samples were collected from wheat fields contaminated with gibberella, and DON degrading bacteria were isolated through enrichment culture; they were identified by morphology and 16S rDNA, gyrB gene sequence. Liquid Chromatography-Tandem Mass Spectrometry and nuclear magnetic resonance were used to identify degradation products, determine degradation pathways, and understand the detoxification mechanisms. The differences between degrading bacteria A4 and DON degrading bacteria A8 and A16 were compared by analyzing strain growth and degradation characteristics, and intergenomic differences. The degradation rate for the DON degrading bacteria, A4, was reported to be 9.7 μg·mLP＞-1P＞ which increased by more than 97% within 10 h. A4 was identified as Devosia sp., that produces 3-keto-DON in the process of degrading DON, thus reducing toxicity. A4 had an optimal growth temperature of 35℃ and an optimal NaCl concentration of 2%. Its heat resistance and salt tolerance were found to be better than those of strains A8 and A16. To degrade DON, A4 requires an optimum pH and temperature of 8.0 and 35℃, respectively. In terms of degradation characteristics, A4 had highter rate than A8 and A16. Comparison between the average nucleotide similarity(ANI)analyses of the three strains showed that the ANI between A4, and A8 and A16 were 81.54% and 77.87%, respectively. The ANI was lower by 95%, indicating that A4 was genetically different from the other two strains, implying it belongs to a different strain. This study has thus screened a novel DON detoxification strain, with good stress resistance, and provides new degrading bacteria resources for DON pollution control. |
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