|
| Identification of ste20-like and comparison with the function of ste20 in Aspergillus flavus |
| Received:November 03, 2022 Revised:November 28, 2022 |
| View Full Text View/Add Comment Download reader |
| KeyWord:Aspergillus flavus;aflatoxin B1（AFB1）;ste20-like;ste20;gene function |
| Author Name | Affiliation | E-mail | | MA Longxue | Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing 100193, China | | | LI Xu | Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing 100193, China | | | XING Fuguo | Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing 100193, China | xingfuguo@caas.cn |
|
| Hits: 1300 |
| Download times: 1481 |
| Abstract: |
| The function of ste20-like in the growth, conidia formation, nucleus formation, and invasiveness was examined, and the differences in these functions with those of ste20 in Aspergillus flavus were determined. Aspergillus flavus NRRL3357 was used in this study. The two genes were compared to identify their similarities and subjected to bioinformatics analysis using NCBI. The target genes were disrupted using homologous recombination. The Δste20, Δste20-like, and double-gene deletion strain(Δdouble)were constructed to examine the effects of gene deletion on the fungal phenotype, sporulation, sclerotia formation, and aflatoxin production. Compared with the wild-type, the growth rate, conidial number, sclerotia number, and pathogenicity were significantly reduced in Δste20, Δste20-like, and the Δdouble strains, showing approximately 76%, 93%, and 72% reductions in aflatoxin B1(AFB1) synthesis, respectively. Comparison of the mutant strains revealed that ste20-like deletion more significantly decreased the growth rate compared to that in Δste20. Conidia and sclerotia production did not significantly differ between the mutant strains. No sclerotia were detected in the Δste20 strain, whereas very few sclerotia were observed in the Δste20-like strain. AFB1 production in Δste20-like was significantly lower than that in Δste20 and the double-gene deletion strain. ste20-like plays important roles in positively regulating mycelia growth, conidia production, sclerotia formation, and AFB1 biosynthesis in A. flavus. Compared with ste20, ste20-like regulates the growth of A. flavus and has a more significant effect on AFB1 synthesis in this species, whereas ste20 has stronger regulatory effects on nucleus formation. |
|
|
|
