|
| Trichoderma harzianum HNA12 and its application in controlling aflatoxin contamination in maize |
| Received:October 24, 2022 Revised:November 22, 2022 |
| View Full Text View/Add Comment Download reader |
| KeyWord:maize;mytotoxin;contamination;wood fungus;biological control |
| Author Name | Affiliation | | ZHANG Lifang | Jiangsu Food and Pharmaceutical Science College, Huai' an 223003, China | | XU Yini | Jiangsu Food and Pharmaceutical Science College, Huai' an 223003, China | | CAO Yang | Jiangsu Food and Pharmaceutical Science College, Huai' an 223003, China | | LIU Liqiang | Hebei University of Science and Technology, Shijiazhuang 050018, China |
|
| Hits: 1284 |
| Download times: 1816 |
| Abstract: |
| To control mycotoxin contamination from the source, high throughput screening and biological control of antagonistic microorganisms against aflatoxin contamination of maize were carried out in this study. In total, 1 055 strains of Trichoderma were isolated from healthy maize seeds collected from Jiangsu, Shandong, Shanxi and Henan provinces. Among them, 10 isolates showed antagonistic activity with a band width of more than 8 mm, and the inhibition rate was ranged from 36.5% to 90.0%. Vivo experiments showed that 7 Trichoderma strains had potent inhibitory effects against A. flavus with a rate of 44.2% to 87.0%, and the inhibition rate against aflatoxin ranged from 40.5% to 85.0%. Among the strains tested, the control efficiency of HNA12, T40-3, and T85-5 against A. flavus was 87.0%, 68.8%, and 64.5%, respectively. The strains with the highest inhibition rates of 85.0%, 75.2%, and 68.5% against aflatoxin were HNA12, T21-1, and T40-3, respectively. Field trial results showed that HNA12 had the highest growth promotion and aflatoxin inhibition rates of 10.5% and 90.5%, respectively, indicating a great potential for biocontrol. Based on morphological and molecular biological analyses, strain HNA12 was identified as Trichoderma harzianum. Antagonistic mechanism test showed that the fermentation product and the protein extract of the strain HNA12 had a strong inhibition ability on the growth of A. flavus, and it could be used as a microbial agent with broad application prospect. |
|
|
|
